产品名称initiocell梯度和细胞间相互作用芯片,IC-DDI-Chip,C-chip: Invasion Chemotaxis chip,C-chip,Invasion Chemotaxis chip,入侵趋化性芯片,3D Cell Culture Chip - Distance Dependent Interaction DDI-Chip,DDI-chip: Distance dependent interactions chip,DDI-chip,Distance dependent interactions chip,iFL-chip: Flow chip,iiF-chip: Immuno-fluorescence chip,iRN-chip: Reaction chip
品牌initiocell
产品货号initiocell梯度和细胞间相互作用芯片,IC-DDI-Chip,C-chip: Invasion Chemotaxis chip,C-chip,Invasion Chemotaxis chip,入侵趋化性芯片,3D Cell Culture Chip - Distance Dependent Interaction DDI-Chip,DDI-chip: Distance dependent interactions chip,DDI-chip,Distance dependent interactions chip,iFL-chip: Flow chip,iiF-chip: Immuno-fluorescence chip,iRN-chip: Reaction chip
产品价格现货询价
联系人李先生
联系电话18618101725
产品说明

initiocell梯度和细胞间相互作用芯片-现货

我们开发用于3D细胞培养的芯片实验室产品和检测方法。 我们专注于梯度和细胞间相互作用。

一、C-chip: Invasion Chemotaxis chip入侵趋化性芯片

确定细胞向3D矩阵的侵袭和迁移
在健康和疾病状态(例如胚胎发育和癌症)中,细胞迁移和侵入3D矩阵都很重要。 IC芯片提供了易于使用且与生理相关的微环境,可以以高时空分辨率直接可视化并定量评估细胞的迁移和侵袭。
用料
含有适当补充剂(MC)的细胞培养基
没有任何补充的细胞培养基(M0)
表达荧光蛋白(GFP,RFP等)或用荧光跟踪器标记的细胞
提供以下染料:
C2925 CellTracker?绿色CMFDA
C34552 CellTracker?CellTracker?红色CMTPX
C34565 CellTracker?深红色
水凝胶,如基质胶(好减少生长因子),胶原蛋白,嘌呤
wu菌H20
镊子处理显微镜载玻片
P20微量移液器,P200微量移液器
带玻璃盖的wu菌显微镜载玻片染色罐(水平)


二、DDI-chip距离依赖性相互作用芯片

The DDI-Chip is a microfluidic device through which the interaction between cells and factors can be investigated at different distances from each other. This provides a more physiological setup and allows simultaneous investigation of different distances in the same device. With the DDI-Chip it is also possible to test agents that can affect the distance dependent interactions.

 

Materials

The DDI-Chip is made of biocompatible polydimethylsiloxane (PDMS) and is gas-permeable to ensure the CO2 and O2 exchange. The support in thin glass is a microscope slide with excellent optical properties such as high transparency and low autofluorescence. This allows a direct observation of your biological sample with temporal and spatial resolution by using optical, fluorescent and confocal microscopes.

 

 

Technical features 

The pattern consists in:

  • 2 medium channels (the external ones) for nutrient supply and tested compounds injection;
  • 2 matrix channels (the intermediate ones) for cell seeding with or w/o hydrogels;
  • 1 main central channel with an width that increases progressively along its length 

Side Medium Channels Intermediate Matrix Channels Middle Channel
Height 150 ?m 150 ?m 150 ?m
Width 2500 ?m

1125 ?m (the narrow parts)

3375 ?m (the wide parts)

Gradually changed from 1250 ?m to 5000 ?m

Volume (recommended for injection)

20 ?L 20 ?L 20 ?L
Diameter of inlets 1000 ?m 1000 ?m 1000 ?m

 

The DDI-Chip has not coated surfaces. According to your application and to eventually improve cell adhesion, you can coat the channel surface by using your solutions of polymers and/or proteins. 

This microfluidic device can be used also with cells embedded in hydrogels such as Collagen I, Collagen IV, Fibrin, Matrigel, etc. to recreate a reliable biological microenvironment

The inlet diameter of 1 mm allows an easy injection of the cell suspension through common pipettes. 20 ?L is the recommended volume for each channel/chamber; this will allow to have few extra ?L for a practical injection and complete filling.


Additionally, it would be possible to perform studies in dynamic flow conditions by using 1/16” tubing directly fitted in the inlets. 

 

Benefits

  • Easy-to-use
  • Physiologically relevant microenvironment 
  • Single cell resolution 
  • Direct visualization
  • Temporal and spatial resolution 
  • Qualitative and quantitative assessments