三维灌流培养系统大全

型号:内详
价格:请致电:010-67529703
品牌:意大利ske,kirkstall品牌,cellecbiotek品牌

一、意大利SKE品牌InFlow多通道三维细胞组织灌注流生物培养系统





1.系统介绍 
InFlow 系统是一套细胞流体灌流生物反应系统,采用可控、双向、间质灌流,通道多达9个,并且相互独立,方便用户对实验的设计,减少了交叉污染的可能性,系统配有pH和O 2光学传感器,可以实时检测实验过程中的pH和培养基中的O2浓度。系统所有材料都具有生物兼容性且均经过无菌处理,并且与培养箱兼容,应用范围广泛,尤其适合骨、心脏组织圆柱形片段的长期培养。





2.特点介绍 

(1). 系统配有9个灌流通道,并且每个都相互独立 

(2). 系统可以双向灌注 

(3). 用户可自定义流量,方向和时间 

(4). 系统不同的型号支架,用户可根据需求选取,操作灵活 

(5). 流速控制范围广泛,0.1 - 6.0 ml/min范围内均可使用,并可精确控制 

(6). 应用范围广泛,统特别适合骨、心脏组织圆柱形片段的长期培养 

(7). 可以设置多组实验,每次可以单独停止一条灌流通路。 

(8). 系统配有光学,非侵入性传感器,可以对pH和培养基中的氧浓度进行实时监控。 

(9). 所有的材料都具有生物兼容性且均经过无菌处理。 

3.应用范围 

InFlow 系统采用可控、双向、间质灌流,通道多达9个,并且相互独立,且系统所有材料都具有生物兼容性且均经过无菌处理,应用范围广泛,是普通流体研究的理想系统,另外特别适合骨、心脏组织圆柱形片段的长期培养。

应用案例:Effect of Perfusion Culture System on In Vitro Osteogenesis of Human Mesenchymal Stem Cells seeded on Porous Hydroxyapatite

通过激光共聚焦扫描显微镜观察,灌流培养比静态培养得到更好的骨骼组织,且没有改变细胞的活性和扩增能力。 

激光共聚焦扫描显微镜检测结果显示灌流培养比静态培养的细胞组织中含有更高的骨钙蛋白的量,产生更均匀、更真实的骨骼组织。 

文献:Saino E, Bloise N, Spinelli L,Effect of Perfusion Culture System on In Vitro Osteogenesis of Human Mesenchymal Stem Cells seeded on Porous Hydroxyapatite.

4.主要参数 

培养小室无菌内部放入支架,支架尺寸: 

支架尺寸:8 [mm] x h 2 [mm] 灌流直径: 6 [mm]

8 [mm] x h 4 [mm] 灌流直径6 [mm]

10 [mm] x h 2 [mm] 灌流直径 8[mm]

10 [mm] x h 4 [mm] 灌流直径 8[mm]

12 [mm] x h 2 [mm] 灌流直径 10[mm]

12 [mm] x h 4 [mm] 灌流直径10 [mm]

培养基储液瓶: 

进口(倒钩接头) 

出口(倒钩接头) 

采样/培养基换端口(鲁尔锁定接头) 

0,22 um过滤器端口(鲁尔锁定接头) 

蠕动泵: 

流速:0.1 - 6 [ml/min]

管道尺寸:ID 1/32"

最多可连接9通道,可有控制器控制 

控制器: 

配备3种不同的控制器:ACE,基于PC版和基于定时器的版本。 

光学传感器: 

配备pH和O 2光学传感器,通过串联连接在液流回路。




5.参考文献 

(1). InFlow Modular perfusion bioreactor--

(2). AutoFeed Automatic medium exchanger

(3). Saino E, Bloise N, Spinelli L, Mantero S, Martinetti R, Imbriani M and Visai L. Effect of Perfusion Culture System on In Vitro Osteogenesis of Human  Mesenchymal Stem Cells seeded on Porous Hydroxyapatite. TERMIS EU meeting Granada 2011.

(4). M. Scavone, N. Bloise, E. Saino, L. Spinelli, L. Fassina, S. Mantero, R. Martinetti, L. Visai. Three-Dimensional Perfusion culture of osteosarcoma cell line (SAOS-2) by bidirectional flow. SIB 2010 Camogli

二、kirkstall品牌Quasi Vivo Cell Culture Flow Systems,Quasi Vivo三维灌流培养系统

Quasi Vivo? Cell Culture Flow Systems,Quasi Vivo三维灌流培养系统

型号:Quasi Vivo
价格:请致电:010-67529703
品牌:kirkstall

Quasi Vivo® Cell Culture Flow Systems


TRL, now Lonza, is the sole distributor of the Quasi Vivo® Interconnected Cell Culture System in the United States and 

Canada. After ten years of research, Kirkstall Ltd.  released the Quasi Vivo® advanced cell culture system to provide ‘in-vivo’ like conditions for cell growth. By providing flow of cell culture media the system provides a more physiologically accurate representation than cultures grown in static well plates.

The Quasi Vivo® system consists of a series of cell culture chambers connected through dynamic flow of media throughout the system. This arrangement allows more in-depth studies of interactions between cell types, and supports meso-scale culture systems to provide more physiologically relevant  metabolic data not available with conventional in-vitro techniques.

Introducing…Quasi Vivo® – Advanced Interconnected Flow Systems

Watch the video below for a 5-minute introduction to Quasi Vivo®, a commercially available Milli-fluid interconnected cell culture system that is flexible, easy to use and provides a significantly more human relevant research environment.





Relevant Industries
  • Pharmaceutical
  • Biotechnology
  • Chemical
  • Cosmetic
  • Academic Research
Benefits
  • Delivers oxygen and nutrients to the cells
  • Removes waste products
  • Stimulates the cells (flow stress)
  • Provides a better mechanism for cell to cell    signaling
  • Enables systemic (multi-organ) models
  •  


    Webinar

    Watch the archived webinar “Building Better In Vitro Models Using the Quasi Vivo® System”, for a proper introduction to Quasi Vivo®. Dr. Kelly Davidge, Kirkstall, discusses the many applications of Quasi Vivo®, with a particular focus on Liver, Cardio and Co-culture models

    WATCH THE ARCHIVED WEBINAR


    Quasi Vivo® Products

    QV500

    The QV500 cell culture chamber provides a flexible research tool for low-flow perfusion cell culture. Molded from medical grade silicone, the QV500 is compatible with monolayer cultures using glass and plastic coverslips as well as a range of  scaffolds to support 3D cell culture models. 

     

    QV600

    The QV600 Air-Liquid Interface (ALI)  chamber has been designed to culture skin, respiratory epithelium, and other tissues requiring membrane support using commercially available standard 24 well hanging inserts.  The QV600 can also be adapted to create a double cavity chamber to study membrane and barrier models. 

     


    QV900

    The QV900 6-chamber optical tray is a compact, disposable unit which provides a range of configuration options within the footprint of a standard well-plate. The QV900 tray is made of cell culture treated polypropylene and each chamber is the diameter of a standard well of a 24-well cell culture plate. 

    Pumps

    TRL can supply two quality peristaltic pumps ideal for working with theQuasi Vivo® system:

      • Parker Polyflex 6-channel peristaltic pump: 6 parallel channel peristaltic pump. Compatible with humidified incubators, manual control.  Power supply accepts any voltage 100-240V.
      • Parker Polyflex 2-channel peristaltic pump: 2 independently controlled pump heads, compatible with humidified incubators, manual controls, dimensions (cm) h:10.5, w 19.5, d 17. 100-120V power.

    Accessories

    We carry the components necessary to replace used parts.

    REQUEST A QUOTE/INFO

    Quasi Vivo® Product Catalog

    QuasiVivo® Catalog Number Description
    QVCWSK Quasi Vivo® QV500 Culture-well starter kit (inc. 3 QV500 chambers, tubing, and reservoirs)
    QVCW5X 5-pack of Quasi Vivo® QV500 culture-well chambers
    QVTWSK Quasi Vivo® QV600 Trans-well Starter Kit (inc. 3 QV600 chambers, tubing, and reservoirs)
    QCTW5X 5-pack of Quasi Vivo® QV600 trans-well chambers
    QV6WSK Quasi Vivo® QV900 6-well starter kit (inc. 3 Quasi Vivo® QV900 6-well trays, tubing, and reservoirs)
    QVT12X 12-pack of Quasi Vivo® QV900 6-well trays
    QVT24X 24-pack of Quasi Vivo® QV900 6-well trays
    QVT60X 60-pack of Quasi Vivo® QV900 6-well trays
    QV6WTU Sterile connecting tubing for Quasi Vivo® QV900 6-well trays
    QVPP6C Parker Polyflex 6-channel peristaltic pump
    QVPP2C Parker Polyflex 2-channel peristaltic pump
    QVRESB Reservoir Bottle

    Only Available in North America.

    Visit the Lonza e-store to place your order online or contact Customer Service at (800)638-8174.

    Quasi Vivo® User Manuals

    Each manual contains detailed instructions on the use of the corresponding product.

    QV500 User Manual

    QV600 User Manual

    QV900 User Manual

    Parker PF 600 Cell Culture Tray User Manual

    Additional Resources

     

    “Why Quasi Vivo®?”
    A series of short videos with Quasi Vivo® users to find out why they use our interconnected flow system within their research.

    A poster that demonstrates how the Quasi Vivo system improves gene expression for many cell types, including hepatocytes.




    三、cellecbiotek品牌u-cup三维灌流培养系统,U-CUP perfusion bioreactor,3D细胞组织灌注培养生物反应器系统

    简单介绍
    U-CUP灌注生物反应器是一种用户友好的工具,用于建立和控制您的3D细胞和组织培养。 U-CUP专门设计用于在生命科学相关领域工作的任何科学家或实验室技术人员使用,而不必要求使用生物反应器的任何先前经验。 应用 器官型模型(骨重建,肿瘤微环境) 3D细胞扩增和分化 细胞 - 支架相互作用的研究 细胞外基质相互作用的研究 生成适合临床前实验的3D细胞支架结构
    产品描述
    cellecbiotek代理,u-cup三维灌流培养系统,U-CUP perfusion bioreactor
    U-CUP灌注生物反应器是一种用户友好的工具,用于建立和控制您的3D细胞和组织培养。
    U-CUP专门设计用于在生命科学相关领域工作的任何科学家或实验室技术人员使用,而不必要求使用生物反应器的任何先前经验。

    应用:

    器官型模型(骨重建,肿瘤微环境)
    3D细胞扩增和分化
    细胞 - 支架相互作用的研究
    细胞外基质相互作用的研究

    生成适合临床前实验的3D细胞支架结构


    cellecbiotek代理,u-cup三维灌流培养系统,U-CUP perfusion bioreactor

    cellecbiotek代理,u-cup三维灌流培养系统,U-CUP perfusion bioreactor,3D细胞组织灌注培养生物反应器系统

    cellecbiotek代理,u-cup三维灌流培养系统,U-CUP perfusion bioreactor,3D细胞组织灌注培养生物反应器系统

    cellecbiotek代理,u-cup三维灌流培养系统,U-CUP perfusion bioreactor,3D细胞组织灌注培养生物反应器系统


    The U-CUP perfusion bioreactor is a user-friendly tool to establish and control your 3D cell and tissue cultures.


    U-CUP has been specifically designed to be used by any scientist or lab technician working in the life science related field, without necessarily requiring any previous experience with the use of bioreactors. 

    Applications
    • Organotypic models (Bone remodeling, Tumor microenvironment)

    • 3D cell expansion and differentiation  

    • Investigation of cell-scaffold interactions  

    • Investigation of cell-extracellular matrix interactions  

    • Generation of 3D cell-scaffold constructs suitable for preclinical experimentation

    Apply instantly your current cell culture concepts and simply let U-CUP further extend them by performing the seamless transition to the 3D context.

    Features Advantages Benefits
    Direct perfusion Unifrom cell seeding  Uniform tissue  
    Efficient nutrition and waste removal  Viable tissue, up to several weeks of culture 
    Physiological conditions (mimicking inetrstitial fluid flow and associated induced shears Physiologically relevant tissue
    Simple and smart design (patented) Easy and ready to use No previous experience with 3D cell cultures required 
    Minimized manual operations Highly reproducible results 
    Efficient with many cell types Versatile cell and tissue culture models 
    Supple scaffold adaptors Compatible with a wide spectrum of 3D porous scaffolds of various composition, architecture and sizes  
    Access to cell culture medium through valves Suitable to seed and co-culture several cell types, even at different culture time points   Possibility to investigate complex cell-cell and cell-extracellular matrix interaction 
    Efficient cell retrieval from scaffolds after culture (with standard enzymatic treatment)  Easy cell analyses (cytofluorimetry, gene expression etc.) 


    Product Configuration

    1x syringe pump

     

    1x rack

      10x U-CUP disposable bioreactor kits  

    1x Starter kit

    cellecbiotek代理,u-cup三维灌流培养系统,U-CUP perfusion bioreactor,3D细胞组织灌注培养生物反应器系统 + cellecbiotek代理,u-cup三维灌流培养系统,U-CUP perfusion bioreactor,3D细胞组织灌注培养生物反应器系统 + cellecbiotek代理,u-cup三维灌流培养系统,U-CUP perfusion bioreactor,3D细胞组织灌注培养生物反应器系统 = cellecbiotek代理,u-cup三维灌流培养系统,U-CUP perfusion bioreactor,3D细胞组织灌注培养生物反应器系统
     The driving force of the system. It generates the oscillating fluid flow of the cell/medium suspension. It cannot be purchased separately.   A rotating rack for easy and correct positioning of U-CUP disposable bioreactors. It can also be purchased separately.   The central core of the system. It is disposable and it comes with 10x adaptors to fit the specific size of your scaffolds. Scaffolds can also be purchased separately.   It provides all what you need to start your 3D cell cultures. Additional accessories (e.g. forceps, syringes) and testing units are also included.

    The performance of U-CUP has been extensively validated and certified by scientific publications in peer-review journals.

    If you are convinced of the benefits that a 3D culture environment can provide,the U-CUP bioreactor is the essential tool to conduct with your experiments.

    For assistance and advice to set up your experiment, do not hesitate to contact CELLEC’s expert team to address your questions.

    应用文献:


    • Boccardo and Gaudiello 2016 In this paper, the perfusion-based bioreactor is used for the generation of an adipose mesenchymal stromal cells -based engineered constructs (Title: Engineered mesenchymal cell-based patches as controlled VEGF delivery systems to induce extrinsic angiogenesis, Acta Biomaterials)

    • Cerino 2016 presents an application for engineering an in vitro 3D multi-cellular muscle-like tissue model (Title: Three-dimensional multi-cellular muscle-like tissue engineering in perfusion-based bioreactors, Biotechnology and Bioengineering)

    • Hirt and Papadimitropoulos 2015 demonstrates the importance of perfusion flow in 3D cultures of tumor cells to efficiently mimic functional features observed “in vivo” and to test anticancer compounds (Title: Bioreactor-engineered cancer tissue-like structures mimic phenotypes, gene expression profiles and drug resistance patterns observed in vivo,Biomaterials)

    • Centola 2015 In this study, the perfusion-based bioreactor system is used to improve cartilage digestion, resulting in higher and more reproducible yield of cell populations with high proliferation and chondrogenic capacity (Title: An improved cartilage digestion method for research and clinical applications, Tissue Engineering Part C, Methods)

    • Bao 2015 presents a humanized in vitro model that reduces the need for experimental animal models, while recapitulating key biological events in a periodontal pocket (Title: Establishment of an oral infection model resembling the periodontal pocket in a perfusion bioreactor system, Virulence)

    • Papadimitropoulos 2014 presents an efficient expansion method of mesenchymal stromal cells by direct seeding and culturing fresh bone marrow preparation within the pores of 3D porous scaffold (Title: Expansion of human mesenchymal stromal cells from fresh bone marrow in a 3D scaffold-based system under direct perfusion, PLoS One)

    • Hirt 2014 highlights the potential of perfusion-based models to create 3D tumour microenvironment for cancer immunobiology studies and pre-clinical assessment of innovative treatments (Title: In vitro 3D models of tumor-immune system interaction, Advance Drug Delivery Review)

    • Papadimitropoulos 2013 presents an application/method for seeding open porous rapid prototyped polymeric scaffolds (Title: A collagen network phase improves cell seeding of open-pore structure scaffolds under perfusion, Journal of Tissue Engineering and Regenerative Medicine)

    • Sadr 2012 presents an application/method to generate a decellularized cell-laid extacellular matrix which enhances the biological performance of polymeric materials (Title: Enhancing the biological performance of synthetic polymeric materials by decoration with engineered, decellularized extracellular matrix, Biomaterials)

    • Gueven 2011 presents an application for upscaling osteogenic and vasculogenic grafts (Title: Engineering of large osteogenic grafts with rapid engraftment capacity using mesenchymal and endothelial progenitors from human adipose tissue, Biomaterials)

    • Papadimitropoulos 2011 presents an application for engineering an in vitro bone organ model (Title: A 3D in vitro bone organ model using human progenitor cells, European Cell & Materials)

    • Di Maggio 2011 a review for our approaches to engineering in 3D vitro niches (Title: Toward modeling the bone marrow niche using scaffold-based 3D culture systems, Biomaterials)

    • Santoro 2010 presents an application for upscaling cartilaginous grafts (Title: Bioreactor based engineering of large-scale human cartilage grafts for joint resurfacing, Biomaterials)

    • Scherberich 2007 presents an application for generating osteogenic and vasculogenic grafts (Title: Three-dimensional perfusion culture of human adipose tissue-derived endothelial and osteoblastic progenitors generates osteogenic constructs with intrinsic vascularization capacity, Stem Cells)

    • Wendt 2006 describes the system for maintaining living uniform tissues in the scaffolds (Title: Uniform tissues engineered by seeding and culturing cells in 3D scaffolds under perfusion at defined oxygen tensions, Biorheology)

    • Braccini 2005 presents an application for generating osteogenic grafts (Title: Three-dimensional perfusion culture of human bone marrow cells and generation of osteoinductive grafts, Stem Cells)

    • Wendt 2003 describes the principle of the U-CUP and its impact on cell seeding (Title: Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity, Biotechnology and Bioengineering)