1、Dynamic Cell Confiner

DYNAMIC CONFINER

A step towards mimicking the natural cell environment

• Control the confinement speed

  Accurate control of the confinement speed through the vacuum pump
• Reversible confinement: retrieve your cells after confinement

  Allows molecular analysis thanks to the non-destructive method for the cells
• Compatible with your own experiments

  The confiner is a small size device that is directly placed on the top of your cell culture

The dynamic cell confiner is a device that allows confining cells within two surfaces with micrometer precision. The space between the two surfaces is controlled by micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston (suction cup). The piston is controlled with a vacuum pump, and the height of the confinement is thus controlled as well. The device can be used in a petri dish, in a glass substrate, etc.

2、6-Wells Static Confiner (CO2 plug)

EXPLORE CELL MECHANICS

6-WELLS STATIC CONFINER WITH CO2 PLUG – CSOW 620

• Define the thickness and shape of cells 

  Control the thickness of your cells with the right confinement slide
• Multiple experiments simultaneously

  Enables the study of different cells or to apply different confinement conditions at the same time
• Adaptable with high resolution microscopy

  Optically transparent materials and compact design enable high resolution microscopy

The CSOW 620 – static confiner is a portable device that allows confining cells within two surfaces with nanometer precision. The space between the two surfaces is controlled by using micro PDMS pillars. The micro pillars are fabricated in a glass slide, which is attached to a PDMS piston. The CSOW 620 is the device that controls the position of the pistons and it is compatible with several brands of 6-well plates.  In the end of the confinement experiments, the cells can be retrieved enabling further biochemical studies. The user decides when and where to confine the cells as it is not necessary to plug it to any other device. In particular, the CSOW 620 is characterized by its compact design and CO2 plug.

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• Features
• Applications
• Example of use

Overall dimensions of the CSOW 620:

Scheme of the CSOW 620:

The static cell confiner CSOW 620 kit is composed by:

• 1 x CSOW 620
• 12 x Confinement Piston in PDMS
• 12 x Confinement Slides – 16 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement

Available confinement heights – from 1, 2, 3, … up to 20 um (you can choose up to 3 heights to integrate your kit)

If you want to make your slides adhesive to your cells or not (optional):

• Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
• Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides

 

If this device does not fit with your constraints, get in touch with us! We can personalize it for you!

3、1-Well Static Confiner