SMART?BCA蛋白质分析试剂盒

型号:21071
联系人:
联系电话:18210996806
品牌:intron



基于BCA测定法优化20~2000 ug / ml蛋白质纯化

  • ?在BSA的工作范围内显示高线性度:20~2,000ug / ml
  • ?即使存在大多数离子/非离子洗涤剂,也可以进行稳定的蛋白质定量
  • ?根据蛋白质类型的变化低于其他方法
  • ?方便用于微孔板(96孔板)
  • ?简单且高灵敏度的蛋白质定量

SMART? BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid(BCA) for the colorimetric detection and quantitation of total protein. The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. This water-soluble complex exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range. (20-2,000 ug/ml) The macromolecular structure of protein, the number of peptide bonds and the presence of four particular amino acids(cystein, cystine, tryptophan and tyrosine)are reported to be responsible for color formation with BCA. Studies with di-, tri- and tetrapeptides suggest that the extent of color formation caused by more than the mere sum of individual colorproducing functional groups. Accordingly, protein concentrations generally are determined and reported with reference to standards of a common protein such as bovine serum albumin (BSA). A series of dilutions of known concentration are prepared from the protein and assayed alongside the unknown(s) before the concentration of each unknown is determined based on the standard curve.


提出了两种测定方法。其中,试管程序需要更大体积(0.1ml)的蛋白质样品; 但是,因为它使用样品与工作溶液的比例为1:20(v / v),干扰物质的影响最小化。微孔板程序使得微孔板的样品处理容易,并且需要较小体积(10-25ul)的蛋白质样品; 然而,由于样品与工作溶液的比例为1:8(v / v),因此在克服干扰物质浓度和获得低水平检测方面提供的灵活性较低。

应用
  • 01亲和层析后测量柱级分
  • 02研究蛋白质:蛋白质相互作用
  • 03估计细胞提取物中膜蛋白的回收百分比
  • 04融合蛋白的高通量筛选
套件内容
内容 试管:200次试验
微孔板:2,000次试验
解决方案A. 250ml×2 ea
解决方案B. 10ml×1 ea
BSA溶液(2 mg / ml) 1ml×10 ea
技术数据

灵敏度比较实验

SMART?BCA蛋白质分析试剂盒比竞争产品灵敏15%至20%。用微孔板读数器(在37℃温育30分钟)测量显色反应。

高保真度

SMART?BCA蛋白质分析试剂盒比竞争对手更敏感,具有相似的变异模式。

A组,SMART?BCA蛋白质分析试剂盒; B组,竞争对手