Athens Research & Technology;16-16-011609;Alpha 1 Antitrypsin, Human Plasma

型号:16-16-011609
联系人:程园
联系电话:18611717737
品牌:athens research & technology


Athens Research & Technology 公司位于美国乔治亚州雅典市,从1986年以来一直致力于纯化高纯度,高活性的人类蛋白质和开发这些蛋白的多克隆抗血清。我们的常规产品包括丝氨酸蛋白酶、蛋白酶抑制剂、中性粒细胞酶、载脂蛋白、脂蛋白,血小板蛋白、转铁蛋白、免疫球蛋白等。二十年多年来,世界各地的研究人员都在使用我们公司的产品。在炎症、冠状动脉疾病、自身免疫性疾病、癌症、老年痴呆症等等研究的主要科学出版物中都能看到引用我们公司的产品。
我们提供优质,可靠的产品和出色的客户服务。我们期待着您的业务。如果需要定制研究服务,Athens Research & Technology 公司能够根据您的项目要求提供无与伦比的试剂。
产品特色
多种天然提取的人类蛋白质产品;多种兔抗人类蛋白质抗血清。



Synonym: α1-Proteinase inhibitor, A1PI, AAT

MW: 52,000
Extinction Coefficient: 0.433

Lyophilized from 30 mM Na phosphate, pH 6.5, with 300 mM NaCl

Storage: -20°C

Purified Native Human Alpha 1 Antitrypsin, Human Plasma
Bulk Qty Available.

An acute-phase plasma protein found at 95-350 mg per 100 ml that functions as a protease inhibitor. Clinically, its deficiency is associated with two major diseases: pulmonary emphysema and early onset/juvenile hepatic cirrhosis. It is elevated in inflammatory conditions, malignancies, liver disease and pregnancy and also after surgical trauma and use of oral contraceptives. The simultaneous quantitative determination of alpha-1-PI and ceruloplasmin permits differential diagnosis of liver afflictions.

Activity: When tested with active-site titrated porcine pancreatic trypsin using Na-Benzoyl-L-Arginine-para-Nitroanilide Hydrochloride (L-BAPNA) as substrate, it is 75-100% inhibitory.

Purity: >=95% by SDS-PAGE

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

Athens Research & Technology products are laboratory reagents and are not to be administered to humans or used for any drug purpose. 
For research use or for use in further manufacturing.

Product Citation: 
Sinden, Nicola J., and Robert A. Stockley. "Proteinase 3 activity in sputum from subjects with alpha-1-antitrypsin deficiency and COPD." European Respiratory Journal 41, no. 5 (2013): 1042-1050.

Borel F, Tang Q, Gernoux G, Greer C, Wang Z, et al.
Survival advantage of both human hepatocyte xenografts and genome edited hepatocytes for treatment of α-1 antitrypsin deficiency.
Molecular Therapy (2017), doi: 10.1016/j.ymthe.2017.09.020

Tang Q, Gruntman AM, Flotte TR. 
Quantification of Total Human Alpha-1 Antitrypsin by Sandwich ELISA. 
Methods Mol Biol. 2017;1639:211-216. doi: 10.1007/978-1-4939-7163-3_20.

Garratt LW, Sutanto EN, Ling KM, Looi K, et al. 
Alpha-1 Antitrypsin Mitigates the Inhibition of Airway Epithelial Cell Repair by Neutrophil Elastase.
Am J Respir Cell Mol Biol. 2016 Mar;54(3):341-9. doi: 10.1165/rcmb.2015-0074OC.

Jansen BC, Falck D, de Haan N, Hipgrave Ederveen AL, Razdorov G, Lauc G, Wuhrer M.
LaCyTools: A Targeted Liquid Chromatography-Mass Spectrometry Data Processing Package for Relative Quantitation of Glycopeptides.
J Proteome Res. 2016 Jul 1;15(7):2198-210. doi: 10.1021/acs.jproteome.6b00171. Epub 2016 Jun 17.

Sinden NJ, et al.
α-1-Antitrypsin variants and the proteinase/antiproteinase imbalance in chronic obstructive pulmonary disease.
Am J Physiol Lung Cell Mol Physiol. 2015 Jan 15;308(2):L179-90.

Ramadass M, Ghebrehiwet B, Kew RR.
Enhanced recognition of plasma proteins in a non-native state by complement C3b. A possible clearance mechanism for damaged proteins in blood.
Mol Immunol. 2015 Mar;64(1):55-62. doi: 10.1016/j.molimm.2014.10.022. Epub 2014 Nov 15.

O'Dwyer CA, et al.
The BLT1 Inhibitory Function of α-1 Antitrypsin Augmentation Therapy Disrupts Leukotriene B4 Neutrophil Signaling.
J Immunol. 2015 Oct 15;195(8):3628-41. doi: 10.4049/jimmunol.1500038. Epub 2015 Sep 14.

Ungurs MJ, Sinden NJ, Stockley RA.
Progranulin is a substrate for neutrophil-elastase and proteinase-3 in the airway and its concentration correlates with mediators of airway inflammation in COPD
Am J Physiol Lung Cell Mol Physiol. 2014 Jan 1;306(1):L80-7. doi: 10.1152/ajplung.00221.2013. Epub 2013 Nov 1.

Sinden NJ, Koura F, Stockley RA.
The significance of the F variant of alpha-1-antitrypsin and unique case report of a PiFF homozygote.
BMC Pulm Med. 2014 Aug 7;14:132. doi: 10.1186/1471-2466-14-132.

Ramadass M, Ghebrehiwet B, Smith RJ, and Kew RR.
Generation of Multiple Fluid-Phase C3b:Plasma Protein Complexes during Complement Activation: Possible Implications in C3 Glomerulopathies.
J Immunol. 2014 Feb 1;192(3):1220-30. doi: 10.4049/jimmunol.1302288. Epub 2013 Dec 23.