3D细胞培养板,三维细胞培养板,三维悬滴培养板,3D细胞悬滴板

型号:内详
价格:请致电:010-67529703
品牌:内详


如今,3D培养技术能制作出能高度模拟体内天然组织的复杂结构,生物化学与药物治疗研究正在向3D培养转变。动物模型花费昂贵,也无法模仿人体内效应;传统2D体外模型相对便宜且容易获取,但毕竟生长在硬梆梆平面上的单层细胞不能模拟天然组织的3D结构,预测体内反应的能力较差。


在已能提供快捷、简便又成熟的3D培养技术的今天,为何还去选择陈旧的平面培养方式!我公司代理多种3D细胞产品,总有一款适合您! 


一、美 国3D Biomatrix公司生产 Perfecta3D®三维细胞培养悬滴板-现货供应




美 国3D Biomatrix公司生产的Perfecta3D®悬滴板是一个新的三维细胞培养平台,能够在多孔板模式中形成大小均匀的球状体悬滴;这种悬滴是细胞自组装形成的球状细胞簇,是三维(3D)细胞培养和筛选中研究的最有利的模型之一。同其他类型的3D支架和水凝胶等方法相比,这种球状体具有简单性,可重复性以及生理组织的相似性,能模拟组织肿瘤固有的代谢(氧气,二氧化碳,养分,废物)和增殖梯度变化。作为优良的生理肿瘤模型可以提供比2D细胞培养更可靠 的结果。

3D Biomatrix提供96孔和384孔的细胞培养板和3D细胞基质(细胞支架)。我们的3D细胞培养能提供与体内相似的结果,极大促进生命科学研究和药物的研发及应用。其产品特点:

1、标准的培养板模式

2、能形成大小均一的球体

3、与高通量筛选仪器兼容

4、易于从培养板的顶端进行维护

5、适合长期培养

6、减少培养基试剂的消耗

7、易于将培养的细胞所分泌出的细胞因子和生长因子分离



订货信息:


96-WELL HANGING DROP PLATES---停产无货

384-WELL HANGING DROP PLATES---现货


二、瑞士inpshero 品牌 3D细胞悬滴培养 ——现货   


瑞士insphero公司生产的3D细胞悬滴培养板无需使用支架介质(scaffold-free),通过悬滴培养法(hanging-drop) ,就能将细胞悬液(一种细胞或多种细胞)形成3D微组织,3D微组织呈多细胞球状体,无论在形态学上,还是在功能上均与自然组织类似。从而更好地模拟体内细胞生长情况,更直观的反映细胞生物学和功能,更准确的构建靶组织模型。 

 

3D细胞培养板主要由GravityPlus板和GravityTRAP板组成,如下图:

 

 

 

 GravityPlusTM板:采用细胞悬滴法(专利技术),使细胞形成球体状微组织(3D状态);将细胞悬液(一种细胞或多种细胞)添加到GravityPlusTM板中,2-4天,细胞在GravityPlusTM板中会形成微组织(微球状);

GravityTRAPTM板:采用非粘附包被技术,使微组织在无依附、不解聚情况下培养数周,便于后续实验及检测。当微组织形成后,将其转移到GravityTRAPTM板中,可使微组织在无依附、不解聚情况下培养数周,便于后续实验及检测。  

 

 

应用实例

 

 

3D细胞悬滴培养板产品优势及应用

 

1. 3D悬滴培养板形成的3D微组织呈多细胞球状体,无论在形态学上,还是在功能上均与自然组织类似。
2. 无需支架介质,细胞可立体生长达数周,可对微组织多次用药
3. 可用于标准化学发光和荧光检测,可检测ATP、LDH、GSH和细胞凋亡等指标
4. 96孔板,精确的尺寸控制,孔间形成的微组织差异甚小,与高通量加样设备兼容,可用于高内涵分析(high content test)
5. 可用于几乎任何类型细胞的3D培养,如细胞系、原代细胞、干细胞及多种细胞共培养
6. 可用于癌症研究及药物分析,药物代谢动力学(DMPK),毒性测试(ADME-TOX)等  
7. 如今,全球最大的前15家制药和护肤品企业均在使用InSphero的GravityPLUS?平台及3D微组织。产品质量符合ISO 9001:2008标准。
 

 

订购信息

 

3D悬滴培养板│GravityPLUS platform kit(货号:CS-06-001,规格10×96孔板

3D悬滴培养板│GravityPLUS? and GravityTRAP? Trial Pack(货号:CS-00-020,规格1×96孔板

3D悬滴培养板│Introductory GravityPLUS and GravityTRAP pack(货号:CS-06-010,规格3×96孔板)

Insphero公司简介 

InSphero是通过类器官式3D微组织培养(supplier of organotypic, biological in vitro 3D microtissues)开展新药测试的领军型开发商。当前全世界最知名的前十家药物和护肤品制造商产品测试都使用到InSphero的专利微组织技术。
    InSphero 3D Insight?微组织用于化学品应用的效能和毒理体外测试,包括长期效应和炎症介导的毒性效应。开发有可供选择的多种肿瘤和肝脏微组织产品及毒理测试模型(toxicology models in the pipeline)。InSphero 3D微组织较传统细胞模式更具有预测能力,培养持续时间更长,价格也更便宜。
    InSphero由Jan Lichtenberg博士, Jens M. Kelm博士和Wolfgang Moritz博士等于2009年成立,是瑞士苏黎世联邦理工学院(Swiss Federal Institute of Technology (ETH) Zurich)和苏黎世大学(University Zurich)的衍生公司,总部设在Schlieren,在美国和德国设有分支机构。InSphero产品获多项国家和国际科学和商业化荣誉,产品质量符合ISO 9001:2008标准。
    InSphero研发团队聚合在微组织工程,实验室自动化和微流体学的50多年丰富经验,带给客户新药测试上全新的、更具洞悉力的体验。


 

三、美国n3dbio 磁悬浮3D细胞培养---现货


美国n3D Biosciences,Inc 以磁化细胞培养技术为基础,研发出一系列磁力驱动3D细胞培养产品。 

磁化细胞培养技术:以NanoShuttle(一种生物相容性磁性纳米颗粒)为中心,将其加入到细胞或培养基中磁化细胞,然后再使用磁力驱动器,就可以让细胞进入磁悬浮状态或将细胞打印(printing)成具有结构和生物性能的典型3D模型(环状或微球状)。 

磁力驱动3D细胞培养产品优势

1. n3Dbio微组织球打印产品与其它微组织球培养装置相比,最显著的优点是速度快:可在极短的时间内(最短达15min)将细胞打印形成微组织球。要求的细胞数量最少能低至625个;细胞数量与3D微组织球尺寸大小间重现性好。 

2. 无需特殊装置、特殊培养基或其它特殊物质;NanoShuttle对细胞无副作用,不改变细胞活力、增殖力,不引起炎症应激反应;不影响观测和成像,不干扰荧光等;细胞可回收,继续WB、qPCR等手续实验。 

3. 想获得大的3D微组织,可选用培养皿/6孔板/24孔板磁悬浮试剂盒;高通量操作,高内涵分析实验,可选用96孔板/384孔板磁力打印试剂盒。

4.  设定iPod即可用于自动成像 ,iPod实验助手软件可自动数据分析 。 

 

产品订购信息

 

产品分类

产品货号

产品名称

试剂盒内容

磁悬浮产品

Bio-Assember

004-SWK

Single Well Bio-Assembler Kit

磁悬浮3D细胞培养皿试剂盒

1.600ul NanoShuttleTM-PL2管)

2.单孔磁力器(6个)

3.35mm皮氏培养皿(10块)

4.100mm的皮氏培养皿(1块)

007-6WK

6-well Bio-Assembler Kit

磁悬浮3D细胞6孔板试剂盒

1. 600ul NanoShuttleTM-PL2管)

2.6孔磁力器(1个)

3.超低吸附6孔板(2

009-24WK

24-well Bio-Assembler Kit

磁悬浮3D细胞24孔板试剂盒

1. 600ul NanoShuttleTM-PL2管)

2.24孔磁力器(1个)

3.超低吸附24孔板(2块)

4.插入式塑料盖(1块)

磁力3D生物打印产品

Bio printing

011-96WK

96-well Bioprinting Kit

96孔板生物打印试剂盒

1. 600ul NanoShuttleTM-PL3管)

2.96球状磁力器(1个)

3.超低吸附96孔板(2块)

012-384WK

384-well BioPrinting Kit

384孔板生物打印试剂盒

1. 600ul NanoShuttleTM-PL2管)

2.384孔球状磁力器(1个)

3.超低吸附384孔板(2块)

活力检测与成像系统

Bio Assay

020-BIOCMSYS

96-Well BiO Assay – Complete System

活力检测与成像全系统

1. 600ul NanoShuttleTM-PL6管)

2.6孔磁力器(1个)

3.96孔球状磁力器(1个)

4.96孔环状磁力器(1个)

5.96孔深孔混匀槽(1个)

6.超低吸附6孔板(2块)

7.超低吸附96孔板(2块)

8. n3Dock(包括灯箱、电池、平板灯)

020-BIOBSCSYS

96-Well BiO Assay – Bioprinting Only

活力检测与成像—生物打印

1. 600ul NanoShuttleTM-PL3管)

2.6孔磁力器(1个)

3.96孔球状磁力器(1个)

4.96孔环状磁力器(1个)

5.96孔深孔混匀槽(1个)

6.超低吸附6孔板(2块)

7.超低吸附96孔板(2块)

020-NSBIOIPD

6-Pack NanoShuttleTM-PL with FREE iPod

6NanoShuttleTM-PL免费的苹果iPod

1. 600ul NanoShuttleTM-PL6管)

2.同时购买020-BIOCMSYS赠送ipod


对于96孔板试剂盒来说,磁力驱动器有两种:Dot磁力驱动器和Ring磁力驱动器,根据培养的细胞类型和应用来选择:

 

 

 n3dbio的辅助产品:Magpen(磁力笔),可用于转移3D微组织或者是用于多细胞分层共培养

四、德国cellendes 水凝胶3D细胞培养——现货



3D 仿生水凝胶系统 ——德国cellendes
3D Life Biomimetic Hydrogel System

 

  

3D 仿生水凝胶系统是一套用于3D细胞培养时构建细胞外基质组分的试剂


使用者可以选择不同polymer和crosslinker组合,构成不同的3D细胞培养

 注:  蓝色:Polymer    绿色:linker    红色:RGD肽 

 

1. polymer 的选择,决定了是否可人为的回收细胞;

2. rosslinker的选择决定了细胞是否能够降解局部水凝胶来为自己创造移动空间;

3. 加RGD多肽可以为细胞提供可粘附的细胞外环境,这也是绝大多数细胞扩散和迁移的先决条件。
 

 3D仿生水凝胶优势及应用

1.Life水凝胶系统与活体细胞外基质相似,采用该系统可使体外细胞培养更接近体内的生理特征,是基础研究、药物筛选和再生医学等领域细胞功能研究的理想选择。

2.3D Life仿生水凝胶不仅适于研究上皮囊和细胞球体等功能性细胞团块,也同样是研究细胞扩散和迁移的理想产品。

3.完全透明的,细胞可在凝胶内或上面培养,可实时观察细胞动向,亦可原位固定,可荧光标记和显微观察。

4.对细胞无任何毒害,选择可降解材料,可以轻松回收细胞

 

3D仿生水凝胶应用实例

 

 

 

产品目录 

 

货号

英文名称

中文名称

规格

09-G-001

3-D Life PVA-PEG Hydrogel Kit

3D细胞聚乙烯马来酰亚胺聚乙二醇仿生水凝胶试剂盒

1 Kit

G81-1

3-D Life PVA-CD Hydrogel Kit

3D细胞聚乙烯马来酰亚胺聚乙二醇、多肽交联物仿生水凝胶试剂盒

1 Kit

G90-1

3-D Life Dextran-PEG Hydrogel Kit

3D细胞葡聚糖马来酰亚胺-聚乙二醇仿生水凝胶试剂盒

1 Kit

G91-1

3-D Life Dextran-CD Hydrogel Kit

3D细胞葡聚糖马来酰亚胺-聚乙二醇、多肽交联物仿生水凝胶试剂盒

1 Kit

L50-1

3-D Life PEG-Link

3D细胞聚乙二醇交联剂

200 ?l

L60-1

3-D Life CD-Link

3D细胞聚乙二醇、多肽交联剂

200 ?l

M80-3

3-D Life Maleimide-PVA Set

3D细胞聚乙烯-马来酰亚胺套装

1 Set

M90-3

3-D Life Maleimide-Dextran Set

3D细胞葡聚糖-马来酰亚胺套装

1 Set

09-P-001

3-D Life RGD Peptide

3D细胞精氨酸-甘氨酸-天冬氨酸肽

1 ?mol

09-P-003

3-D Life Scrambled RGD Peptide

3D细胞精氨酸-甘氨酸-天冬氨酸肽

1 ?mol


 

五、美国3D Biotek 品牌 3D细胞培养支架


六、 SCIVAX Life Sciences NanoCulture Plate  三维细胞培养板

NanoCulture Plate 以其独特的纳米印记技术,为细胞提供三维生长环境,促进细胞与细胞之间的成团,减少细胞与容器间的贴壁结合,有利于保持细胞的体内状态,在形态、增殖、基因表达、代谢及功能等特性上与体内细胞更加接近。
NanoCulture Plate 的主要应用:
(1)辅助药物候选物的筛选,尤其是抗癌药物的细胞水平的筛选;三维细胞球体药物测试结果能更可靠的预测药物体内测试结果。
(2)建立有效可靠的细胞模型,进行体外的药物毒性、代谢测试以及药效测定。
(3)有效促进干细胞的分化,提高干细胞分化效率
(4)RNAi筛查
(5)细胞信号传导、细胞相互作用研究,生物标记的发现。


NanoCulture Plate 的特点


(1)孔板底部为印有纳米纤维的薄膜,具备良好的透光性,三维培养过程中不需要添加任何干扰光线的成分,可进行清晰的
       细胞成像及高灵敏的荧光测定。
(2)纯化学聚合物材料,不含任何蛋白或动物源成分,具备良好的质量稳定性,可常温储存和运输。
(3) 操作方面与常规二维细胞培养一样简便,易于操作和掌握,提高了实验的可重复性。
(4) 所有的细胞球体分布在同一水平高度,可同一焦距观察所有的孔,因此可实现自动化的高通量筛选。








NanoCulture Product Listings

Category

Product Name

Quantity

Category #

NCP/NCD

NanoCulture Plate MS Pattern, Low-Binding, 384-well

10 plates

NCP-LS384-10

NCP/NCD

NanoCulture Plate MS Pattern, Low-Binding, 384-well

100 plates

NCP-LS384-100

NCP/NCD

NanoCulture Plate MS Pattern, Low-Binding, 96-well

2 plates

NCP-LS96-2

NCP/NCD

NanoCulture Plate MS Pattern, Low-Binding, 96-well

10 plates

NCP-LS96-10

NCP/NCD

NanoCulture Plate MS Pattern, Low-Binding, 24-well

2 plates

NCP-LS24-2

NCP/NCD

NanoCulture Plate MS Pattern, Low-Binding, 24-well

10 plates

NCP-LS24-10

NCP/NCD

NanoCulture Plate MS Pattern, High-Binding, 384-well

10 plates

NCP-HS384-10

NCP/NCD 

NanoCulture Plate MS Pattern, High-Binding, 384-well 

100 plates

NCP-HS384-100

NCP/NCD

NanoCulture Plate MS Pattern, High-Binding, 96-well

2 plates

NCP-HS96-2

NCP/NCD

NanoCulture Plate MS Pattern, High-Binding, 96-well

10 plates

NCP-HS96-10

NCP/NCD

NanoCulture Plate MS Pattern, High-Binding, 24-well

2 plates

NCP-HS24-2

NCP/NCD

NanoCulture Plate MS Pattern, High-Binding, 24-well

10 plates

NCP-HS24-10

NCP/NCD

NanoCulture Plate MH Pattern, Low-Binding, 384-well

10 plates

NCP-LH384-10

NCP/NCD

NanoCulture Plate MH Pattern, Low-Binding, 384-well

100 plates

NCP-LH384-100

NCP/NCD

NanoCulture Plate MH Pattern, Low-Binding, 96-well 

2 plates

NCP-LH96-2

NCP/NCD

NanoCulture Plate MH Pattern, Low-Binding, 96-well

10 plates

NCP-LH96-10

NCP/NCD

NanoCulture Plate MH Pattern, Low-Binding, 24-well

2 plates

NCP-LH24-2

NCP/NCD

NanoCulture Plate MH Pattern, Low-Binding, 24-well

10 plates

NCP-LH24-10

NCP/NCD

NanoCulture Plate MH Pattern, High-Binding, 384-well

10 plates

NCP-HH384-10

NCP/NCD

NanoCulture Plate MH Pattern, High-Binding, 384-well

100 plates

NCP-HH384-100

NCP/NCD

NanoCulture Plate MH Pattern, High-Binding, 96-well

2 plates

NCP-HH96-2

NCP/NCD

NanoCulture Plate MH Pattern, High-Binding, 96-well

10 plates

NCP-HH96-10

NCP/NCD

NanoCulture Plate MH Pattern, High-Binding, 24-well

2 plates

NCP-HH24-2

NCP/NCD

NanoCulture Plate MH Pattern, High-Binding, 24-well

10 plates

NCP-HH24-10

NCP/NCD

NanoCulture Plate 96-well trial kit (MS/MH pattern, Low-Binding)

2 plates (1 each)

NCP-LSH96-2

NCP/NCD

NanoCulture Plate 24-well trial kit (MS/MH pattern, Low-Binding)

2 plates (1 each)

NCP-LSH24-2

NCP/NCD

NanoCulture Dish MS Pattern, Low-Binding, 35mm

5 dishes

NCD-LS35-5

NCP/NCD

NanoCulture Dish MS Pattern, High-Binding, 35mm

5 dishes

NCD-HS35-5

NCP/NCD

NanoCulture Dish MH Pattern, Low-Binding, 35mm

5 dishes

NCD-LH35-5

NCP/NCD

NanoCulture Dish MH Pattern, High-Binding, 35mm

5 dishes

NCD-HH35-5


七、Microtissues无支架的3D培养系统



MicroTissues Inc. is a privately held company advancing technologies and applications of 3D cell culture. The company’s products stand above the rest because they are designed to create more natural and more reliable 3D cell culture environments based on cell-to-cell interactions in convenient and consistent formats that generate high content information. The company’s lead line of products, the 3D Petri Dish®, is serving the needs of researchers in a wide range of areas including cancer research, stem cell biology, toxicity testing, developmental biology, drug discovery, regenerative medicine and tissue engineering. In addition to products for basic research, MicroTissues Inc. is using its platform technology to pursue applications in drug discovery and cell therapy.


3D Petri Dish®





    

The 3D Cell Culture Technology
The 3D Petri Dish® is the natural 3D cell culture environment that is scaffold-free and that maximizes cell-to-cell interactions.

 Useful for spheroids, mammospheres, gliomaspheres, hepatospheres, chondrospheres, osteospheres, cell aggregates, neurospheres, cardiospheres, embryoid bodies and myoballs, we sell you the micro-molds to cast your own 3D Petri Dish® out of agarose and these gels fit in standard 12 and 24 well dishes. The micro-molds are autoclavable and reusable, so you save thousands of dollars on your 3D cell culture experiments. You also realize significant new technological advantages:

?Make hundreds of spheroids of uniform size in a single pipeting step.
?Control spheroid size.
?Image an array of spheroids on the same optical plane.
?Form spheroids of two different cell types.
?Grow spheroids from single cell clones.
?Harvest spheroids for RT-PCR and Western Blots without enzymes.
?Form microtissues in complex shapes for novel applications.
Cells seeded on the 3D Petri Dish® used for 3D Cell Culture
When cells are seeded onto the 3D Petri Dish®, cell-to-cell adhesion drives the self assembly of the 3D microtissue, typically in 1-2 days. No synthetic scaffold to alter cell physiology, no variability due to ill defined gels, no animal products. 3D cell culture in a format suitable for high content microscopy that has worked with over twenty different cell types both primary cells and cell lines.



订货信息:


货号

产品

Z764086

MicroTissues® 3D Petri Dish® micro-mold honeycombs makes honeycomb-shaped microtissues, fits 24 well plates, honeycomb diam. 3.4 mm

Z764094

MicroTissues® 3D Petri Dish® micro-mold mixed spheroid kit 3 small and 3 large, fits 12 well plates

Z764108

MicroTissues® 3D Petri Dish® micro-mold mixed spheroid kit 3 small and 3 large, fits 24 well plates

Z764027

MicroTissues® 3D Petri Dish® micro-mold rods fits 12 well plates, 4 x 15 array

Z764078

MicroTissues® 3D Petri Dish® micro-mold rods fits 24 well plates, 3 x 8 array

Z764000

MicroTissues® 3D Petri Dish® micro-mold spheroids size S, 16 x 16 array, fits 12 well plates

Z764019

MicroTissues® 3D Petri Dish® micro-mold spheroids size L, 9 x 9 array, fits 12 well plates

Z764043

MicroTissues® 3D Petri Dish® micro-mold spheroids size S, 8 x 12 array, fits 24 well plates

Z764051

MicroTissues® 3D Petri Dish® micro-mold spheroids size L, 5 x 7 array, fits 24 well plates

Z764116

MicroTissues® 3D Petri Dish® micro-mold Tech Evaluation kit 1 of each mold

Z764035

MicroTissues® 3D Petri Dish® micro-mold toroids makes toroid-shaped microtissues, fits 12 well plates, 6 x 6 array


八:RealBio D4? Culture System



Single Use Disposable
Simple and easy to use. The system arrives pre-sterilized and ready for your next experiment. Simply attach the flow loop and begin.  

Perfusion
Culture media is perfused tangentially across the scaffold (red arrows),  by a standard peristaltic pump. This facilitates complete tissue density through out the scaffold.

Independent Gassing

Oxygen is delivered independent from media, by gassing chambers on top and bottom (blue arrows). This reduces shear force and enables a more in-vivo like environment by creating gradients of nutrient and oxygen concentrations.