Orflo代理 Moxi Z迷你自动细胞计数仪,MOXI FLOW 掌上迷你微型流式细胞仪 MXC001,MXC002,MOXI Z Mini Automated Cell Counter Kit

型号:Moxi Z ,MOXI FLOW
价格:请致电:010-67529703
品牌:orflo代理

一、Orflo品牌Moxi Z迷你自动细胞计数仪及芯片-现货促销

简单介绍
Moxi Z是一款由美国Orflo公司开发的细胞计数仪,它是第一款手持式细胞计数器,它采用库尔特电阻抗原理以及Orflo专利的薄膜传感器技术对细胞进行计数及直径测量。Moxi Z无需任何染料即可轻松对平均直径范围在3-25um区间的细胞、酵母进行精确计数。计数结果以直方图的形式来显示细胞的直径和体积,提供细胞密度和细胞分布数据。整个计数过程能够在8-15秒内完成。
产品描述

 

Moxi Z 微型自动细胞计数仪(ORFLO第一款手持式细胞计数器)

备有现货促销

Moxi Z简介

Moxi Z是一款由美国Orflo公司开发的细胞计数仪,它采用库尔特电阻抗原理以及Orflo专利的薄膜传感器技术对细胞进行计数及直径测量。Moxi Z无需任何染料即可轻松对平均直径范围在3-25um区间的细胞、酵母进行精确计数。计数结果以直方图的形式来显示细胞的直径和体积,提供细胞密度和细胞分布数据。整个计数过程能够在8-15秒内完成。

 

 

细胞计数更可靠、更便宜、更方便

Moxi Z这款高端的细胞计数仪,综合了两种方法:细胞计数的金标准——库尔特原理【亦称电阻法、电脉冲法与电感应区技术,因其属于对颗粒个体的测量和三维的测量,不但能准确测量物料的粒径分布,更能作粒子绝对数目和浓度的测量。】以及ORFLO开发的专利薄膜传感器技术,无需任何辅助试剂,即可轻松计数。还提供了一个标准化的评估活细胞/死细胞的专用软件,用于生成Moxi活力指数(MVI)算法报告。
这项**性的超小体积的仪器旨在克服手工计数的乏味、相对于基于图像的计数系统缺乏重复性和流式细胞仪高昂的价格。Moxi Z能够对直径2-34 μm的细胞进行计数,适用于哺乳动物细胞、血小板、酵母及其他微生物。由于检测下限为2μm,也就避免了漏掉一些细胞的风险。
Moxi Z
是一款真正物超所值的自动细胞计数仪。不仅计数准确,仪器成本低,后续消耗也非常小,是做细胞培养客户的首选。

 

 

技术参数:

√细胞大小:直径2-34微米

√测量时间:8秒

√浓    度:3,000至500,000细胞/ml

√分辨率:1200直方图bins

√活力评估:MVI(Moxi Viability Index)

√数据存储:500直方图

√连    接:蓝牙连接Mac / PC

√电    池:2300mAh锂离子

√交流电源:100 - 240V,50/60Hz,0.2A

√重    量:0.67kg

√尺    寸:19.0cm长x 10.8cm宽x 7.0cm高

系统特点:

√Moxi Z速度快、精准,非常坚固,没有维护成本

√仪器稳定,不用校正

√不用辅助试剂,芯片是唯一的消耗品,使用成本低

√“智能技术”的仪器,可自动检测已插入芯片是使用过的或新的

√芯片覆盖直径范围(2-34微米)内的所有细胞样品,无需根据细胞大小进行更换

√电池寿命长

 

·    工作原理

电子电流通过细胞传感区(在薄膜上的一个小孔)。当有细胞通过时,电压会瞬时增加,并电压的改变与细胞或颗粒的大小成正比。细胞单个依次流过细胞传感区时,测量每个细胞的大小并以直方图形式保存。总容积数是由精确测量的流体体积进行分析。
操作

自动测量,仅需8秒钟。

注:每个芯片可做2个测试。当样品1完成后,只需取出,将芯片的另一端插入Moxi Z,加入样品2
数据处理

每次测量,一个完整的直方图显示,以及细胞计数,细胞大小和平均Moxi活力指数(MVI)。可在Moxi Z查看结果或将结果上传到电脑上利用随机软件做额外的分析。培养细胞的健康状况,可通过比较直方图来评估。Moxi Z还可执行复杂的曲线拟合分析,以更准确地确定重叠细胞(或碎片)计数事件,显着改善了测量体积产生的计数精确性。

Mozi Z 可存储500个样品数据。所有数据可以通过USB或蓝牙转移到PC /Mac。不需要特殊的软件,因为数据文件是Excel兼容的。PC / Mac的分析软件也包含在每一个Maxi Z中。
准确计数细胞和测定细胞活力,无需辅助试剂,仅需8
分析细胞培养的两项最重要的可测量的指标:准确的细胞计数和活细胞/死细胞的比例
细胞活力指数--无需辅助试剂测定细胞活力
此前,研究人员推断活细胞或死细胞的计数是基于对某种染料的排斥,或吸收/保留其他种染料。这些方法具有局限性,往往是昂贵的,复杂的,可能需要几分钟才能完成的测定。使用突破性的专有软件算法,MoxiZ提供的细胞活力指数(Moxi Viability IndexMVI),与公认的染料为基础的方法具有高度相关性。仅需8秒,无需任何试剂。上图展示的是MVI数据与广为接受的流式细胞仪和传统的血球计台盼蓝排斥方法的数据比较,证明这两套数据是等值的。

 准确性

凭借其宽广的细胞大小的动态范围和专利技术,MoxiZ提供最高的细胞计数准确性,用于哺乳动物细胞,酵母,藻类和其它一些粒子,而无计数丢失的风险。在与金标准计数方法比较,如图所示,MoxiZ被证明具有相当甚至优于金标准的结果。数据表明,Moxi Z达到宽范围浓度和粒子类型的计数性能。
 
精确度

过去50年,由于最高水平的精确度,Coulter电子技术细胞计数已经普遍被视为细胞计数技术的黄金标准。MoxiZ使用相同的基础技术来实现对细胞的计数。图中数据显示,Moxi Z与明显更大,更昂贵的库尔特计数仪相比具有相同等级的精确度,这样低的差异远远优于基于成像技术的其它品牌计数仪。
 
速度

平均测试时间仅为8秒,Moxi Z是最快的细胞计数技术。

适用范围:

如今,大部分实验室还在利用血球计数器来进行手工计数,耗时费神不说,结果也不准确。若是借助大型的全自动细胞计数系统或者流式细胞仪,那结果自然准确得多,但就是价格不那么可爱。

Moxi Z这款独特的仪器则克服了上面两种方法的缺点,有着高端仪器的性能,但同时小巧易用,价格也便宜得多。Moxi Z综合了两种方法:细胞计数的金标准——库尔特原理以及ORFLO开发的专利薄膜传感器技术,无需任何试剂,即可轻松计数。

Moxi Z在计数时使用一种小巧的cassette,每个cassette能容纳2个样品。独特的设计让样品从两端上样。在cassette插入时,仪器的“智能技术”能自动检测,并检测此cassette是全新的还是用过的。对于所有样品,均使用相同的cassette。

每次测量仅需8秒,仪器就能带来细胞大小、细胞数量以及细胞健康指数(Moxi Viability Index),这些信息对研究人员分析细胞培养情况很重要。Moxi Z能够对直径2-34μm的细胞进行计数,适用于哺乳动物细胞、血小板、酵母及其他。由于检测下限为2 μm,也就避免了漏掉一些细胞的风险。每次测量需要75 μl样品,其中细胞浓度范围在3,000-2,500,000个细胞/ml。

测量完之后,显示屏显示如下。图中不仅包括了细胞数量和平均大小,还有完整的直方图,反映样品中细胞大小的分布。通过直方图的比较,您可以轻松发现细胞培养间的细胞变化。仪器本身可储存500个样品的数据。此外,所有数据也可通过USB接口或蓝牙传输到电脑中

 

Moxi Z的参数如下:

 细胞大小

2-34 μm

测量时间

8

浓度

3,000-500,000个细胞/ml

分辨率

1200 histogram bins

活力评估

Moxi Viability Index

重量

约0.65 kg

尺寸(长×宽×高)

7.5英寸×4.25英寸×2.75英寸

数据储存

500个直方图

交流电源

100-240V,50/60 Hz,0.2 A

连接

USB和蓝牙



Moxi Z Cell Count Cassettes Type S  芯片现货供应



Moxi Z Cell Count Cassettes Type S

Product Code: MXC002

Moxi Z, Cell Count Cassettes, Type S, 25 pack (50 tests)

Higher Performance & Simplicity in One Disposable Cassette.


Patent-protected thin-film cassettes provide highly accurate, repeatable cell counts and cell size analysis in under 8 seconds (15 seconds for Type S cassette). Inspired by the gold-standard Coulter Principle used in high-end counting systems worldwide, the Moxi cassette flows thousands of cells per test through a cell sensing zone to accurately capture the impedance-based volumetric measurement of each individual cell in the sample. Impedance-based cell counting of individual cells is proven to be over 95% accurate versus a 75% accuracy with image-based cell counting methods.

No system contamination and integrated pre-filter for clogging prevention.


Moxi cassettes are designed with a unique ""cell sieve"" to minimize cell clumping and clogging. In addition, Moxi cassettes contain 100% of your sample within the cassette body, eliminating the possibility for system contamination and sterilization. Cassettes are simply and easily discarded when testing is finished.

Two tests per cassette.


Each Moxi cassette offers two individual tests. The system will alert the user if a used cassette is mistakenly inserted in the unit.

Multiple cassette options.


Users can choose from Moxi Type M or Moxi Type S cassettes depending on cell specifications

id MXC002
Average Cell Diameter Range 3 - 20 microns
Cassette Types Type S
Cell Particle Concentration Range 3,000 - 1,750,000 cells/mL
Cell Types Tested HEK-293|HeLa|PC12|CD3+T|CHO-K1|Cos-7|HepG2|Hybridoma|Jurkat E6-1|K562|MCF7|Mesenchymal SC|Monocyte|Mouse ESC| NIH 3T3| PBMC (cultured)|Red Blood Cells (RBC)|L5178y| C. albicans (Yeast)| S. cerevisiae Vin 13 (Yeast)|S. cerevisiae X5 (Yeast)|Wine Yeast (natural fermentaion)|S.cerevisiae (Baker's Yeast|Safale US-05 Yeast|
Compatible Instruments Moxi Z
Intended Use Statement For Research Use Only. Product is not for use in diagnostic procedures
Measurable Dynamic Range 3 - 26 microns
Measurement Time 15 seconds
MPICell Health Ratio Moxi Viability Index MVI Yes
Number of Tests 50
Overall Dimensions 125.7 L x 50.9 W x 27.44 H (mm)
Sample Type Mammalian Cells|Most Yeast|Small Algae|Small Protozoa
Sample Volume 75 L
Sterility Non-sterile
Useable Cell Volume 14 - 9,202 fL
Weight 66.3 g

How It Works

Electronic current is passed through a small hole (Cell Sensing Zone) in a thin-film membrane. Cells flow substantially single file through the CSZ causing momentary increases in measured voltage that are directly proportional to cell or particle volume. Thousands of cells are measured during a single test and the size of each cell is plotted and saved in histogram format. Total volumetric counts are determined by precisely measuring the volume of fluid being analyzed.

Step 1:

Insert cassette into Moxi Z.

Step 2:

Pipette 75 μL sample into cassette. Touch Screen.

Finished! Automatic, hands-free measurement in just 8 seconds.

Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into MoxiZ, and load Sample 2.

Data

For each measurement, a complete histogram is displayed as well as cell count, average cell size and Moxi Population Index. The raw waveform from the most recent cell count, containing each cell spike, is also stored on the Moxi Z and can be both viewed on the Moxi Z and uploaded to the PC for additional analysis. The Moxi Z also performs sophisticated curve fitting analysis to more accurately determine cell counts in the event of overlapping populations of cells (or debris), significantly improving the accuracy of the volumetric counts produced by the Moxi Z. Data for 500 samples may be stored on the unit. All data can be transferred to a PC/Mac via Bluetooth. No special software is required for the PC since date files are Excel®-compatible. Sophisticated PC/Mac compatible analys software is included with each Moxi Z. Cell health may be assessed by comparing differences in histograms from culture to culture.




Moxi Z Cell Count Cassettes Type M 芯片 现货供应

Moxi Z Cell Count Cassettes Type M

Product Code: MXC001

Moxi Z, Cell Count Cassettes, Type M, 25 pack (50 tests)

Higher Performance & Simplicity in One Disposable Cassette.


Patent-protected thin-film cassettes provide highly accurate, repeatable cell counts and cell size analysis in under 8 seconds (15 seconds for Type S cassette). Inspired by the gold-standard Coulter Principle used in high-end counting systems worldwide, the Moxi cassette flows thousands of cells per test through a cell sensing zone to accurately capture the impedance-based volumetric measurement of each individual cell in the sample. Impedance-based cell counting of individual cells is proven to be over 95% accurate versus a 75% accuracy with image-based cell counting methods.

No system contamination and integrated pre-filter for clogging prevention.


Moxi cassettes are designed with a unique ""cell sieve"" to minimize cell clumping and clogging. In addition, Moxi cassettes contain 100% of your sample within the cassette body, eliminating the possibility for system contamination and sterilization. Cassettes are simply and easily discarded when testing is finished.

Two tests per cassette.


Each Moxi cassette offers two individual tests. The system will alert the user if a used cassette is mistakenly inserted in the unit.

Multiple cassette options.


Users can choose from Moxi Type M or Moxi Type S cassettes depending on cell specifications



id MXC001
Average Cell Diameter Range 4 - 25 microns
Cassette Types Type M
Cell Particle Concentration Range 3,000 - 500,000 cells/mL
Cell Types Tested HEK-293|HeLa|PC12|CD3+T|CHO-K1|Cos-7|HepG2|Hybridoma|Jurkat E6-1|K562|MCF7|Mesenchymal SC|Monocyte|Mouse ESC|NIH 3T3|PBMC (cultured)|Red Blood Cells (RBC)|L5178y|S. cerevisiae Vin 13 (Yeast)|S. cerevisiae X5 (Yeast)|Wine Yeast (natural fermentaion)|S. cerevisiae (Baker's Yeast|Safale US-05 Yeast|
Compatible Instruments Moxi Z
Intended Use Statement For Research Use Only. Product is not for use in diagnostic procedures
Measurable Dynamic Range 4 - 34 microns
Measurement Time 10 seconds
MPICell Health Ratio Moxi Viability Index MVI Yes
Number of Tests 50
Overall Dimensions 125.7 L x 50.9 W x 27.44 H (mm)
Sample Type Mammalian Cells|Large Yeast|Large Algae|Protozoa
Sample Volume 75 L
Sterility Non-sterile
Useable Cell Volume 34 - 20,580 fL
Weight 66.3 g

How It Works

Electronic current is passed through a small hole (Cell Sensing Zone) in a thin-film membrane. Cells flow substantially single file through the CSZ causing momentary increases in measured voltage that are directly proportional to cell or particle volume. Thousands of cells are measured during a single test and the size of each cell is plotted and saved in histogram format. Total volumetric counts are determined by precisely measuring the volume of fluid being analyzed.

Step 1:

Insert cassette into Moxi Z.

Step 2:

Pipette 75 μL sample into cassette. Touch Screen.

Finished! Automatic, hands-free measurement in just 8 seconds.

Note: Each cassette holds 2 tests. When Sample 1 is complete, simply re-insert other end of cassette into MoxiZ, and load Sample 2.

Data

For each measurement, a complete histogram is displayed as well as cell count, average cell size and Moxi Population Index. The raw waveform from the most recent cell count, containing each cell spike, is also stored on the Moxi Z and can be both viewed on the Moxi Z and uploaded to the PC for additional analysis. The Moxi Z also performs sophisticated curve fitting analysis to more accurately determine cell counts in the event of overlapping populations of cells (or debris), significantly improving the accuracy of the volumetric counts produced by the Moxi Z. Data for 500 samples may be stored on the unit. All data can be transferred to a PC/Mac via Bluetooth. No special software is required for the PC since date files are Excel®-compatible. Sophisticated PC/Mac compatible analys software is included with each Moxi Z. Cell health may be assessed by comparing differences in histograms from culture to culture.




二、orflo品牌MOXI FLOW 掌上迷你微型流式细胞仪及芯片-现货促销

美国ORFLO公司最新推出的一台手持式微型流式细胞仪。她的外观灵巧,完全可将她托放与掌中,便于移动,让你的实验地点不再被庞大的仪器所束缚;操作便捷,无需受过专业培训就可以完成操作,因为所有操作就只有两步(Plug&Play),上机10秒后就可在彩色触屏显示器上看到实验结果,简便至极。价钱方面,对于绝大多数的实验室来说,如果想拥有她,从经济角度上讲是完全可行的。看到这里估计读者已经迫不及待的想进一步了解和认识她了,下面就由笔者向各位介绍这位灵动、聪慧的姑娘 — MoxiFlow(图1)。 
orflo品牌MOXI FLOW 掌上迷你微型流式细胞仪(MoxiFlow手持式微型流式细胞仪) orflo品牌MOXI FLOW 掌上迷你微型流式细胞仪(MoxiFlow手持式微型流式细胞仪) 
内外兼修
对于它外观的小巧此处不再累述(大小规格仅为26×15×15cm),图片比文字更有说服力(图2);仪器内置4500mAh锂电池,便于在实验室内的移动和随意放置;内置软件程序,480X320pix彩色显示器可清晰呈现数据结果,让您一目了然。至于检测方式,它采用了两种检测的完美融合—库尔特电阻原理与荧光检测原理: 
orflo品牌MOXI FLOW 掌上迷你微型流式细胞仪(MoxiFlow手持式微型流式细胞仪)orflo品牌MOXI FLOW 掌上迷你微型流式细胞仪(MoxiFlow手持式微型流式细胞仪) 
图2 手持式流式细胞仪 
一、 库尔特原理(用于检测细胞大小及数量)
当悬浮于液体中的细胞通过小孔管时,会取代相同体积的液体,在恒定电流设计的电路中导致小孔管内外的电阻发生瞬时变化,产生电位脉冲,而这种脉冲信号的大小和次数与细胞的大小与数目成正比,因此可用于细胞计数及大小的检测。这正是谈到流式细胞仪的历史而不可避开的库尔特原理,库尔特电阻检测法属于对细胞个体的测量和三维的测量,不但能准确测量细胞的大小,更能对细胞的绝对数量及浓度进行测量。利用库尔特原理检测细胞大小更近真实,而不像激光衍射散射原理会受到染料颜色和浓度的影响。流式细胞仪(FCM,Flow cytometry),从字面上的cytometry (细胞计数) 也看得出它与细胞计数有着一定的渊源,Wallace Coulter于1947年发明了库尔特原理,用于血细胞分析,随后1953年Coulter公司便推出了世界上第一台流式细胞分析仪。而以库尔特原理为主导技术开发细胞计数仪的ORFLO公司在此推出这样一台众望所归的微型流式细胞仪也便是名正言顺了,并利用其专利的薄膜传感器技术对细胞进行数量、直径等检测,获取更加精准可靠的数据。 
二、 荧光检测(用于检测荧光信号)
MoxiFlow配置了一根532nm固态激光器和一个PMT,检测光谱区域为590/40nm ( 可检测染料:R-PE, PI, Nile Red, Ethidium Homodimer I & III, Sytox Orange等 )。对细胞大小检测的同时以传统流式的方法利用激光激发标记在细胞上的特异性荧光染料,并通过PMT进行检测,获取荧光信号。 
MoxiFlow 即利用了以上两种检测方式相结合的方式,在细胞流经小孔管时,同时对细胞的大小、数目及荧光信号进行精确的检测(如图3)。 
          orflo品牌MOXI FLOW 掌上迷你微型流式细胞仪(MoxiFlow手持式微型流式细胞仪)                

化 繁 为 简 
一、 简易化操作
微流体系膜片内设细胞过滤功能,所以对于准备上机的细胞悬液无需进行滤网的过滤,直接即可加入膜片孔内准备实验(如图4),此外膜片的窗口处为非透明材质做成,形成小的避光暗室,避免了荧光检测过程中荧光猝灭现象;仅需两步即可完成所有操作步骤。一,将细胞微流膜片插入检测口;二,将50μL样品加入检测孔内,点击Play即可开始检测 (如图5~6);仅10秒钟即可显示出实验结果。输出数据的格式为标准的流式数据格式FCS3.1或.BMP的图像输出格式,结果数据可以以散点图及直方图(如图7)的形式表现出来,并可轻松用手触摸。 
orflo品牌MOXI FLOW 掌上迷你微型流式细胞仪(MoxiFlow手持式微型流式细胞仪) 
二、 程序化实验
MoxiFlow将较为常用的流式实验程序化为软件中的多个选项 (如图8,并在不断更新升级中),在实验室过程中只需点击要进行的实验名称即可完成相应的数据检测,如: 细胞活性检测、 细胞凋亡检、 多参数分析、细胞大小检测、荧光珠子检测、 细胞周期检测(coming soon)等,使用系统内相应实验程序即可,应用哪里,点哪里,无需再对电压、荧光补偿等参数进行调整。没有经过任何专业培训的人员也可轻松操作完成,可见MoxiFlow解放了科研人员对于流式细胞仪使用操作的繁琐性及专业性,是对流式细胞仪的一次崭新的革命。 
orflo品牌MOXI FLOW 掌上迷你微型流式细胞仪(MoxiFlow手持式微型流式细胞仪)   orflo品牌MOXI FLOW 掌上迷你微型流式细胞仪(MoxiFlow手持式微型流式细胞仪)
    
为了展现出MoxiFlow优秀的检测性能,下面分别将MoxiFlow与台式流式细胞仪、成像细胞分析系统、血细胞计数板进行比较。结果发现,对于细胞的计数误差要低于其他类型产品,而计数平均值的变异系数(CV)也是最小的,可见 MoxiFlow对于细胞数量的检测表现优秀(图9 左);对于细胞活性的检测误差值与平均活性检测的波动性(CV)都是最小的,即在对细胞活性检测的实验中,MoxiFlow依然很好地保证了数据的精准度和稳定性(图9 右)。 
orflo品牌MOXI FLOW 掌上迷你微型流式细胞仪(MoxiFlow手持式微型流式细胞仪) 
下图是MoxiFlow细胞凋亡实验的结果图,此实验是用喜树碱(一种抗癌药物)对Jurkat 细胞(白血病细胞)分别刺激不同时间(2h,6h,7h)所得到的散点图。可以评测出该药物对Jurkat细胞凋亡的作用,并给出各个群落的细胞数量、细胞浓度、占细胞总数的百分比及细胞的平均直径等信息。
orflo品牌MOXI FLOW 掌上迷你微型流式细胞仪(MoxiFlow手持式微型流式细胞仪)orflo品牌MOXI FLOW 掌上迷你微型流式细胞仪(MoxiFlow手持式微型流式细胞仪)
总结 
外形的小巧并没有影响MoxiFlow的应用性能,所谓,麻雀虽小五脏俱全。它可帮你摆脱对流式细胞仪“想爱不敢爱”的矛盾心理,让流式不再高不可攀,让细胞在你的掌中流动。