neuroindx品牌Unipick单细胞抓取系统,UnipicK单细胞捕获抓取系统
型号:Unipick,Unipick plus
价格:请致电:010-67529703
品牌:neuroindx

UnipicK单细胞捕获抓取系统

品牌:

NEUROINDX

产品名称:

单细胞和组织挑取系统

产地:

美国

型号:

UnipicK

——多用途和高性价比的细胞采集和组织显微切割系统

产品简介:

  目前仍无实用快速的技术可以用来从细胞培养直接采集感兴趣的单细胞用作下游单细胞分析和再培养。同样,异质性组织采集特异性的组织区域或单细胞仍然具有挑战性。现有的技术,包括流式细胞筛选和激光辅助的技术,都很昂贵和复杂,并且在使用上有各样的局限性。UnipicK是由美国NIH资助的最新的单细胞和组织采集系统,经过5年研发,由美国NEUROINDX研发生产。该系统性能独特,使用专利保护的脉冲负压为动力,玻璃毛细管为工具,可以和大部分倒置显微镜配合使用,可以快速和精确地直接从常规细胞培养板采集单细胞或细胞克隆,并且对细胞活性影响轻微。采集的细胞可以再培养,和用作下游各种单细胞分析。

  由于UnipicK采集后的单细胞仍需要手动通过微小注射器来释放出细胞,所以20171NEUROINDX推出了具有自动采集和自动释放单细胞的新一代单细胞和组织挑取系统:UnipicK Plus。经过莱比信的反复验证,确定其可以自动将采集后的细胞放到旁置的PCR管中,PCR管架具有48孔,可以满足绝大多数客户的需求。

  UnipicKUnipicK Plus系统均可以从细胞培养体系和复杂异质性组织,比如悬浮细胞体系、三维培养的组织体系、脑组织、病理切片等,根据组织病理学,荧光标记和解剖学需要来采集单细胞和特异性的解剖学区域。UnipicKUnipicK Plus采集的样品可广泛用于下游众多领域的现代分子生物学技术,特别是针对蛋白质和核酸,包括但不限于定量 RT-PCR,全基因表达,表观遗传学和蛋白质组学研究等。系统本身较已有的系统相比价格低,一机多用,经济实惠,性价比高,容易使用,简单快捷,耗材便宜,采集成本低。仪器不需要专门的维护,结实耐用。

  一次性毛细管针头直径在15-100μm之间,满足不同大小的细胞的选取

  可供采集毛细管内径大小:15203040506080100 μm

  主要应用领域

  原代组织细胞培养、回收

  干细胞的有效分选研究

  分离人的单克隆抗体

  癌症研究与治疗

  基因组学和蛋白质组学分析

  检测及分离悬浮培养液中的稀有细胞,如肿瘤循环细胞CTC

  生物芯片细胞分选

  单细胞PCR和基因表达分析

  肿瘤干细胞的分离

  动物肿瘤模型分析

  主要特点

  操作简便,容易上手

  单细胞分辨率

  组织显微切割

  可采集贴壁,悬浮和三维细胞培养

  采集的细胞保持高活性

  高品质 RNA和蛋白质用于下游各种组学分析

  灵活性和多用途

  经济实惠,高性价比

  采集成本低

  仪器主要优势

  系统操作:配备高精度机械移动电机,保证微量毛细管位于视场的正中心

  毛细管内径范围:15~100um,可给予客户的应用提供最优的内径选择方案

  组织样品的厚度: 10~300um,可直接从载玻片上进行细胞分选

  针对细胞种类:血液、骨髓、制备的各种组织细胞悬浮液,任何单一类型细胞或、稀有细胞(如循环肿瘤细胞、内皮细胞、干细胞以及血液、骨髓中相关细胞等)

  样品采集过程:不涉及化学,热,激光和辐射处理,对细胞影响很小,保证细胞的活性和完整性,所分离的组织或细胞可以提取高质量的 DNA RNA 和蛋白质,供下游研究使用

  工作效率:一般的分选过程只需几分钟就可完成

  客户反馈

  UnipicK是多用途的产品—可以从细胞培养和组织切片采集样品

  UnipicK具有灵活性—和几乎所有倒置显微镜兼容v

  UnipicK具有无与伦比的回退功能,多孔培养板仍然可以畅通无阻

  UnipicK具有再校对功能—实验过程更高效,更快速

  相关文献

  Xu MJ, Cooke M, Steinmetz D, Karakousis G, Saxena D, Bartlett E, et al. (2015) A Novel Approach for the Detection and Genetic Analysis of Live Melanoma Circulating Tumor Cells. PLoS ONE 10(3): 0123376. doi:10.1371/journal.pone.0123376

  Vi N, Ma Z, Fields T, Avliyakulov NK, Haykinson MJ, Bragin A, and Karsten SL (2012) Novel Cell and Tissue Acquisition System (CTAS): Microdissection of Live and Frozen Brain Tissues. PloS One. 7(7):41564.

  使用客户

  清华大学,武汉大学,浙江大学,复旦大学,同济大学,厦门大学,中山大学,中国科学院有机化学研究所,神经科学研究所,国家蛋白质科学中心,南方科技大学,深圳人民医院转化医学中心,爱尔眼科学研究所,香港城市大学,香港中文大学,澳门大学



UNIPICK

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UnipicK is a vacuum-assisted single cell collection and tissue microdissection instrument based on NeuroInDx’s original capillary-based cell and tissue acquisition system (CTAS; USPT 8,797,644; Kudo et al, 2012) that can acquire individual cells from cell cultures and tissues. It permits rapid acquisition of single cells from any untreated cell cultures, providing reliable and affordable means of most sophisticated experimentation. Moreover, the instrument is suitable for individual cell collection and region specific acquisition of subanatomical areas from complex heterogeneous tissues such as the brain. Operation of the system is intuitive and requires minimal training. Cell cultures require no treatment or specific peripherals, thus enabling unmatched flexibility in experimental design and sample collection.

Perhaps one of the most distinct features is the ability of UnipicK™ to collect live cells for further reculturing and a plethora of downstream functional studies.  Both individual fluorescently labeled and morphologically distinct cells can be acquired from any cell cultures or tissue sections.  The procedure has been demonstrated from nearly all types of adherent, suspended and 3D cultures, including difficult samples such as primary cells, or individual cells from fresh frozen and sucrose treated complex tissues, such as brain (please, seehttp://www.youtube.com/user/NDXInc). With live cells viability tests have demonstrated up to 99% after collection, and up to 30 cells per minute may be collected from adherent cultures.

RNA isolated from collected cells and tissue regions exhibits high integrity and low degradation rates with RIN >9.0. Subsequent labeling protocols (e.g. T7 based) consistently produce high incorporation yields making it suitable for downstream PCR, microarray and sequencing analyses.

The instruments’ low cost, ease of use, single cell resolution, and minimal impact on cell viability, make it unique and vital research tools. Importantly, UnipicK™ is a free standing instrument that can be mounted over a wide range of inverted microscope models, providing additional flexibility in the laboratory.

 

Notable Features of UnipicK™ include:

– Simple operation with minimal training

– Single cell collection from untreated cultures and tissue sections

– Efficient tissue microdissection

– High viability of collected cells

– High quality RNA and protein for downstream –omics studies

– Flexibility and versatility – fit any inverted microscope

– Fraction of the cost of any laser assisted microdissection instrument