5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.

Product Specifications:
5-Fluoroorotic acid monohydrate (5-FOA)
5-Fluorouracil-4-carboxylic acid

MOLECULAR BIOLOGY GRADE

Formula: C₅H₃FN₂O₄ • H₂O

MW: 192.10 g/mol

White to slightly off-white crystalline powder

Storage/Handling: Store at -20°C. Protect from light.

PubChem Chemical ID: 16212749