Fast Maxime™ RT PreMix (Oligo (dT)15 Primer)

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Fast Maxime™ RT PreMix (Oligo (dT)15 Primer)

Cat.No Capacity Inquire
25089 96 tubes Inquire
PRODUCT INFORMATION
Description

Dried & aliquoted RT Premix kit for simple and rapid cDNA synthesis from eukaryotic RNA

  • • Oligo (dT) pirmer is included to synthesize cDNA from various derived RNA
  • • High RT (reverse transcription) efficiency allows rapid synthesis of cDNA at 5 min
  • • All reagents required for the reaction are stored in dried pellet in one tube
  • - cDNA synthesis is possible only by adding template and RNase free water
  • • Excellent performance, reproducibility and stability
  • - Excellent reproducibility with aliquoted premix by ALHP system
  • - Excellent product stability by vacuum compression manufacturing method

Fast Maxime ™ RT PreMix (Oligo (dT) 15 Primer) enables effective cDNA synthesis in a short time (5 min). Using the ALHP system, all components required for synthesis are accurately dispensed into each tube. In addition, it is a dried pellet type product which shows remarkable reproducibility by significantly reducing the error in the experiment. It is a product with excellent stability due to oxidation and moisture prevention of major constituents in product by vacuum compression manufacturing method. This product is easy to apply to RNA with poly (A) tail because it contains Oligo (dT) primer and can be easily tested by adding template RNA and RNase free DW. The synthesized cDNA can be applied to various subsequent experiments and excellent follow-up results are derived.


 

Applications
  • 01Ligation & transformation
  • 02Construction of full length cDNA libraries
  • 03 RT-PCR
  • 04Real-time RT-PCR
Kit Contents
Components Volume
Fast Maxime™ RT PreMix (Oligo (dT)15 Primer) 96 tubes
Manual 1 ea
TroubleShooting Guide
QThe oligo dT primer binds to the poly (A) tail of the mRNA and promotes cDNA synthesis.
ARandom primers are used for mRNA as well as for rRNA, tRNA, and total RNA And then cDNA synthesis is carried out. Therefore, it can be selected according to the purpose of your experiment.
QI used the synthesized cDNA to perform subsequent experiments, but the amount of amplification is very small.
AIt is recommended to check the concentration of template RNA among various causes and increase the amount. It is also recommended to increase the reverse transcription reaction time.
QWhat is the storage period of cDNA synthesized with RT Premix?
AIt is recommended to use synthesized cDNA immediately for subsequent experiments. It can be stored at -70 ℃ for 6 months for long term storage.
QDo I have to use the total volume of 20 μl for RT reaction?
AFast Maxime ™ RT PreMix (Oligo (dT) 15 Primer) is optimized for each component for 20 μl reactions.
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