miRacle™ miRNA Extraction Kit (Dual)

型号:
价格:联系人:王,请致电:010-67529703,手机(微信同):18210996806,QQ:997554969
,品牌:intronbio

miRacle™ miRNA Extraction Kit (Dual)

Cat.No Capacity Inquire
17521 50 tests Inquire
PRODUCT INFORMATION
Description

Spin column type product for extracting total RNA or micro RNA from cell, tissue and blood

  • • Possible to extract high yield and purified miRNA & Total RNA within 40 mins
  • • High reproducibility
  • • Minimizing contamination by CAPS method ( Column)
  • • Possible for downstream applications such as miRNA assay, RT-PCR or qRT-PCR

miRacleTM miRNA extraction kit(Dual) is designed to extract total RNA or micro RNA easily from cell, tissue, blood. This product is possible for extracting large amounts of sample without alcohol PPT procedure. Contamination is minimized by introducing individual packaged system and it is very convenient for performing experiment. One of the biggest advantages of this product is that it can extract only total RNA or micro RNA specifically, depending on the column and method selection. This product is suitable for cDNA synthesis, RT-PCR, real-time RT-PCR, micro RNA assay, Northern Bolt etc. by using the obtained products (total RNA and micro RNA).

1. Spin Column & CAPS Information

Applications
  • 01RT-PCR and real-time RT-PCR
  • 02micro RNA assay
  • 03Northern Blotting and Dot Blotting
  • 04cDNA synthesis
Kit Contents
Components 50 prep
Lysis Buffer 60ml
Washing Buffer A 12ml
Washing Buffer B 15ml
Elution Buffer 15ml
Spin total-Column (O-ring white) 50ea
Spin micro-Column (O-ring yellow) 50ea
Centrifuge Tube 1.5 ml 50ea
Centrifuge Tube 2 ml 100ea
Manual 1 ea
Technical Data

Comparison data for efficiency of total RNA and micro RNA extraction based on various products

 Lane M, DNA Marker; [HeLa cell] Lane 1, Supplier A; Lane 2 : Supplier B; Lane 3, miRacleTM miRNA extraction kit (Dual) (miRNA); Lane 4, miRacleTM miRNA extraction kit (Dual) (Total RNA); [293T cell line] Lane 5, Supplier A; Lane 6. Supplier B; Lane 7, miRacleTM miRNA extraction kit (Dual) (miRNA); Lane 8, miRacleTM miRNA extraction kit (Dual) (Total RNA)

Micro RNA assay based on extracted RNA

 

  

TroubleShooting Guide
Q이 제품을 이용하여 total RNA도 추출이 가능한가요?
AmiRacle™ miRNA extraction kit (Dual) 의 매뉴얼은 Total RNA와 small RNA를 추출 할 수 있게 구성되어 있습니다.
small RNA(miRNA etc.)는 2개의 컬럼[Spin total-Column (O-ring white)과 Spin micro-Column (O-ring yellow)]을 사용하고,
total RNA의 추출은 1개의 컬럼[Spin total-Column (O-ring white)]을 사용합니다.
실험의 목적에 맞는 방법으로 실험을 진행 하시면 됩니다.
Q전기영동 결과, RNA degradation이 의심 됩니다.
ARNA는 DNA보다 쉽게 degradation 될 수 있으므로, 실험시 주의를 기울여야 합니다.
사용하는 모든 기기 및 장비를 알코올 및 락스 세척을 통해 오염원을 최소화 하고 latex glove 및 mask를 착용하고
DEPC가 처리된 멸균된 증류수를 사용하여 실험을 진행해 주시길 바랍니다.
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