ProGlow^TM Western Blot Protein Ladders are designed for Western blots. ProGlow^TM ladders can be used to monitor electrophoresis progress and to visualize protein standard bands directly on X-ray film or digital analytical equipment. ProGlow^TM ladders consist of eight recombinant proteins in the range of 12-90 kDa. The 70 kDa protein is a prestained marker used for monitor of the electrophoresis process and evaluation of Western transfer efficiency. The other seven markers can bind to human, mouse, rat, goat, sheep and rabbit IgG with their IgG binding site, allowing direct visualization of markers on the X-ray film after ECL development.

The ProGlow^TM Western Ladder will give varying signal intensity depending upon the concentration of your primary and secondary antibodies. GoldBio strongly recommends that you perform a test experiment with the ProGlow^TM Ladder to determine the optimal amount to load on your gel. Run a test gel and Western blot in which 5 µl, 1 µl, 0.3 µl, and 0.1 µl of ProGlow^TM Ladder is loaded, and examine the signal intensity.

Storage/Handling: Store at 4°C for 3 months. Store at -20°C for 24 months.
This product is stable for shipping at ambient temperatures.

Directions for Use:
1. Thaw to room temperature, and mix gently.
2. Load 1-5 µl ProGlow^TM Western Blot Ladder. We recommend testing different loading amounts of the
    standards to determine the optimal amount to use under your experimental conditions.
3. After electrophoresis, transfer proteins to a NC or PVDF membrane.
4. Perform the blocking step followed by incubating with primary antibody and secondary antibody.
5. After the thorough washing, incubate membrane with ECL reagent, and then expose the X-ray film in
    the dark room.
6. ProGlow^TM Western Blot Ladder is stable at 4°C for six month. Please put at -20°C for long term
    storage.