Product Overview
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Concentration
1 mg/ml
Description
Rabbit polyclonal antibody raised against a synthetic peptide corresponding to the N-terminus of histone H3 acetylated on K18, ChIP-grade
Purification
Affinity purified
Specificity
Broad Range, Mouse, Rat, Human
Isotype
IgG
Immunogen
A synthetic acetylated peptide corresponding to residues surrounding K18 of human H3
Formulation
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage
Store at -20°C or -80°C. Avoid freeze / thaw cycles.
Alternative Names
H3K18ac antibody; H3K18a antibody;H3t; H3.4; H3/g; H3FT
Application
WB 1:1000 - 1:3000
IHC 1:200- 1:500
IF 1:500- 1:1000
IP 1:200- 1:500
ChIP 1:50- 1:100
