牌品：,产品名称：TASCL (3D mass culture device)，细胞簇培养板，TASCL 3D细胞团微球培养板，TASCL细胞团微球培养装置，TASCL1000，TASCL600，TASCLTM 1000 Well and TASCLTM 600 Well,产品货号：TASCLBrand,TASCLItem#,TASCL (3D mass culture device)，细胞簇培养板，TASCL 3D细胞团微球培养板，TASCL细胞团微球培养装置，TASCL1000，TASCL600，TASCLTM 1000 Well and TASCLTM 600 Well

产品名称	TASCL (3D mass culture device)，细胞簇培养板，TASCL 3D细胞团微球培养板，TASCL细胞团微球培养装置，TASCL1000，TASCL600，TASCLTM 1000 Well and TASCLTM 600 Well
品牌
产品货号	TASCL (3D mass culture device)，细胞簇培养板，TASCL 3D细胞团微球培养板，TASCL细胞团微球培养装置，TASCL1000，TASCL600，TASCLTM 1000 Well and TASCLTM 600 Well
产品价格	现货询价
联系人	李先生
联系电话	18618101725
产品说明 TASCL 3D细胞团微球培养板细胞块(椭球体)大量培养(三维培养)分化诱导微板“TASCL”是以东京大学的微细加工技术和表面处理技术为基础开发的。特长是可以一次大量培养大致均匀形状和大小的细胞块，也可以通过约1个月的长期培养实现分化诱导。使用方法简单，也可以在TASCL上进行细胞的显微镜观察。器材和培养基试剂的总成本也很便宜。 ※研究用途 TASCL?（用于簇培养的锥形模板）微孔板是一种创新的 3D 培养设备，旨在轻松高效地创建同质多细胞 3D 球体或胚状体 (EB)。通过诱导多能干 (iPS) 细胞的广泛测试，TASCL 有望在再生医学和体外药物筛选中发挥关键作用，可提供患者特异性和疾病特异性鲸准医学研究。使用 TASCL 培养的 iPS 细胞产生了包含三个胚层的多细胞球体或 EB。这些 EB 仿生结构和功能比 2D 中的单个细胞更好，使其适用于代谢、毒性和再生医学的研究。关键特性 ? 在一次播种操作中，每口井可批量生产 600-1000 个均质球体。 ? 井的设计允许养分和氧气循环，使其成为长期观察和分化研究的里想选择。 ? 高密度孔设计可减少培养基和试剂的使用。片上球体培养实验室用锥形模板一次培养大量形状和大小均一的细胞团(球状体)。通过长期孵育约1个月可以诱导分化。预处理和灭菌以保护蛋白质。 *基于东京大学的微制造和表面处理技术，设备的底面可以被穿透，气体和介质可以循环细胞培养板及其制造方法(综述) ①可以一次大量培养(三维培养)大致均匀大小的球状细胞块(椭球体) (约3600个/约6000个) ②由于能够长时间保持细胞状态良好的底面贯通，气体和培养基循环 ③由于可以进行分化诱导的1个月的长期培养 ④可削减总成本，采用小、高密度的井结构，节约器材和培养基试剂 ⑤打开后马上可以使用的蛋白质防附着处理完毕，灭菌完毕，预先器材装好 ⑥简单使用的播种只需从上面播种细胞悬液(不需要离心分离)，培养基更换也很容易 ⑦也可以在放置于容易观察的TASCL上的状态下进行细胞的显微镜观察英文介绍： TASCL? (Tapered Stencil for Cluster Culture) microplates are an innovative 3D culture device developed to easily and efficiently create homogenous multicellular 3D spheroids or embryoid bodies (EB). Extensively tested with Induced pluripotent stem (iPS) cells, TASCL is expected to play a crucial role in regenerative medicine and in vitro drug screening that can provide patient specific and disease-specific precision medicine research. iPS cells cultured using TASCL created multicellular spheroids or EB containing three germ layers. These EB biomimic the structure and function better than individual cells in 2D, making them suitable for studies in metabolism, toxicity, and regenerative medicine. KEY Characteristics ? Mass produce 600-1000 homogenous spheroids per well in one seeding operation. ? Design of the well allows for nutrients and oxygen to circulate, making it ideal for long-term observation and differentiation studies. ? High density well design allows for less usage of medium and reagents. TASCL Principles and Technology TASCL is a 3D microfabricated microplate made of silicone rubber with surface treatment technology. Its patented design of dense array of pass-through holes and inclined walls allows for all seeded cells to settle into the holes. In addition, its thin membrane keeps the cells within these holes, but allows for nutrients and oxygen to circulate through. TASCL microplates are provided on a pre-set culture insert and ready-to-use in a 6-well plate. Proof of Concept: Multicellular 3D Spheroids - Embryoid Bodies (EB) [Reference Literature] 1.Yukawa, H. et al. Embryonic body formation using the tapered soft stencil for cluster culture device. Biomaterials 32, 3729–3738 (2011). 2.Miyamoto, Y., Ikeuchi, M., Noguchi, H., Yagi, T. & Hayashi, S. Enhanced Adipogenic Differentiation of Human Adipose-Derived Stem Cells in an In Vitro Microenvironment: The Preparation of Adipose-Like Microtissues Using a Three-Dimensional Culture. Cell Med 9, 35–44 (2017). 3.Miyamoto, Y., Ikeuchi, M., Noguchi, H., Yagi, T. & Hayashi, S. Spheroid Formation and Evaluation of Hepatic Cells in a Three-Dimensional Culture Device. Cell Med 8, 47–56 (2015). 4.Miyamoto, Y., Ikeuchi, M., Noguchi, H., Yagi, T. & Hayashi, S. Three-Dimensional In Vitro Hepatic Constructs Formed Using Combinatorial Tapered Stencil for Cluster Culture (TASCL) Device. Cell Med 7, 67–74 (2015).