双轴三维回转微重力模拟系统

比美国国家航空航天局(NASA)的 RCCS RWV一维微重力旋转仪更优秀经济，因为一维旋转器中很难wanquan消除重力矢量，可能产生离心力

因为一维旋转器中很难wanquan消除重力矢量，可能产生离心力

该系统模拟微重力的是一个同时控制两轴旋转的多向g力发生器。这一du特的功能允许取消累积重力矢量在该设备的中心。这是通过在装置中心旋转一个腔室来实现的，以恒定的角速度将重力矢量均匀地分散在一个球形体中。在该两轴旋转多向微重力系统中培养的霉菌生长为球状而不是地毯状(图1b)。由于该两轴旋转多向微重力系统所产生的微重力环境均匀，更适合对实验参数要求严格控制的生物学研究。

特点：

1、该系统是双轴三维回转微重力模拟系统允许取消累积重力矢量在该设备的中心。这是通过在装置中心旋转一个腔室来实现的，以恒定的角速度将重力矢量均匀地分散在一个球形体中比美国国家航空航天局(NASA)的 synthecon RCCS一维微重力旋转仪更优秀经济，因为一维旋转器中很难wanquan消除重力矢量，可能产生离心力。

由于概统所产生的微重力环境均匀，更适合对实验参数要求严格控制的生物学研究。一个显著的对比是，1D-clinostat加速了msc的软骨细胞分化，而该系统抑制了成骨细胞、成肌细胞和msc的细胞分化。

2、的模块化安装系统 轻松自定义自定义功能利用双电压电源，4k摄像机，LED等增强您的实验设计。

3、转速范围：0.3-15RPM 

4、NoIR成像模块静止图像：3280 X 2464px 

5、NoIR成像模块视频：1080P @ 30fps，480p @ 90fps 

6、照明模块：IRLED，RGBLED，UVLED（正在开发） 

7、传感器模块：9自由度重力/旋转/加速度传感器 

8、双电压电源：5v和12v路由至内核 

9、延长线：长五米 

10、恒温箱安quan：37°C和95％湿度

11、直观，灵活的用户界面控制

12、多种样品夹：

60mm培养皿（成像）
60mm培养皿堆栈
120毫米Petridish（成像）
120mm培养皿堆栈
96孔培养皿
1个T25烧瓶（旋转中心）
12个T25烧瓶（平衡）
1个T75烧瓶（旋转中心）
4个T75烧瓶（平衡的）

应用文献：

• Richard Hilleary, Julio Paez-Valencia, Cullen Vens, Masatsugu Toyota, Michael Palmgren, and Simon Gilroy. Tonoplast-localized Ca2+ pumps regulate Ca2+ signals during pattern-triggered immunity in Arabidopsis thaliana. PNAS

• Srujana Neelam, Brian Richardson, Richard Barker, Ceasar Udave, Simon Gilroy, Mark Cameron, Howard G Levine, Ye Zhang (2020). Changes in nuclear shape and gene expression in response to simulated microgravity are LINC complex-dependent. Molecular biology and space medicine.

• Caleb Fitzgerald, Cullen Vens, Nathan Miller, Richard Barker, Matthew Westphall, Johnathan Lombardino, Jerry Miao, Sarah J. Swanson and Simon Gilroy. Using the Automated Botanical Contact Device (ABCD) to deliver reproducible, intermittent touch stimulation to plants. Methods in Molecular Biology

• Richard Barker, John Lombardino, Kai Rasmussen and Simon Gilroy. (2020) Test of Arabidopsis Space Transcriptome: A Discovery Environment to Explore Multiple Plant Biology Spaceflight Experiments. Frontiers in Plant Science.

• Won‐Gyu Choi Richard J. Barker Su‐Hwa Kim Sarah J. Swanson and Simon Gilroy (2019). Variation in the transcriptome of different ecotypes of Arabidopsis thaliana reveals signatures of oxidative stress in plant responses to spaceflight. American Journal of Botany.

• Masatsugu Toyota, Dirk Spencer, Satoe Sawai-Toyota, Wang Jiaqi , Tong Zhang, Abraham Koo, Gregg Howe and Simon Gilroy (2018). Glutamate triggers long-distance, calcium-based plant defense signaling. SCIENCE.

• Richard Barker and Simon Gilroy (2017). Life in space isn’t easy, even if you’re green. Biochemist 39:6.

• Fitzgerald CP, Barker RJ, Choi W-G, Swanson SJ, Stephens SD, Huber C, Nimunkar AJ, Gilroy S (2016). Development of Equipment that Uses Far-Red Light to Impose Seed Dormancy in Arabidopsis for Spaceflight. Gravitational and Space research.

• Alvarez AA, Han SW, Toyota M, Brillada C, Zheng J, Gilroy S, Rojas-Pierce M. (2016) Wortmannin-induced vacuole fusion enhances amyloplast dynamics in Arabidopsis zigzag1 hypocotyls. Journal of Experimental Botany 67(22):6459-6472.

• Louren?o TF, Serra TS, Cordeiro AM, Swanson SJ, Gilroy S, Saibo N, Oliveira MM. (2016) Rice root curling, a response to mechanosensing, is modulated by the rice E3-ubiquitin ligase HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE1 (OsHOS1). Plant Signaling and Behaviour 11(8):e1208880.

• Evans MJ, Choi WG, Gilroy S, Morris RJ. (2016) A ROS-assisted calcium wave dependent on the AtRBOHD NADPH oxidase and TPC1 cation channel propagates the systemic response to salt stress. Plant Physiology 171:1771-1784.

• Gilroy S, Bia_asek M, Suzuki N, Górecka M, Devireddy AR, Karpi_ski S, Mittler R. (2016) ROS, calcium, and electric signals: key mediators of rapid systemic signaling in plants. Plant Physiology 171:1606-1615.

• Choi WG, Hilleary R, Swanson SJ, Kim SH, Gilroy S (2016) Rapid, long-distance electrical and calcium signaling in plants. Annual Review of Plant Biology 67:287-307.

• Louren?o T, Serra T, Cordeiro A, Swanson S, Gilroy S, Saibo N, and Oliveira M (2015). The rice E3 ubiquitin ligase OsHOS1 modulates the expression of OsRMC, a gene involved in root mechano-sensing, through the interaction with two ERF transcription factors. Plant Physiology, 169: 2275-2287.

• Matthew J. Evans, Won-Gyu Choi, Simon Gilroy, Richard J. Morris. A ROS-Assisted Calcium Wave Dependent on the AtRBOHD NADPH Oxidase and TPC1 Cation Channel Propagates the Systemic Response to Salt Stress. Plant Physiology.

• Choi WG, Gilroy S (2015) Quantification of sodium accumulation in Arabidopsis thaliana using inductively coupled plasma optical emission spectrometry (ICP-OES). BioProtocols Vol 5, Iss 16.

• Swanson SJ, Barker R, Ye Y, Gilroy S (2015) Evaluating mechano-transduction and touch responses in plant roots. Methods in Molecular Biology 1309:143-150.

• Gilroy S, Suzuki N, Miller G, Choi WG, Toyota M, Devireddy AR, Mittler R (2014) A tidal wave of signals: calcium and ROS at the forefront of rapid systemic signaling. Trends in Plant Science 19: 623-630.

• Jayaraman D, Gilroy S, Ané JM. (2014) Staying in touch: mechanical signals in plant-microbe interactions. Current Opinions in Plant Biology 20C:104-109.

• Choi WG and Gilroy S (2014) Plant Biologists FRET over stress. Elife 15:e02763.

• Swanson SJ and Gilroy S (2014) Gravitropism. In: eLS. John Wiley & Sons Ltd, Chichester.

• Choi WG, Toyota M, Kim S-H , Hilleary R and Gilroy S (2014) Salt stress-induced Ca2+ waves are associated with rapid, long-distance root-to-shoot signaling in plants. PNAS 111:6497-6502.

• Toyota M, Ikeda N, Sawai-Toyota S, Kato T, Gilroy S, Tasaka M and Morita MT (2013). A new centrifuge microscope reveals statolith movements underlie gravity sensing in Arabidopsis shoots. The Plant Journal, 76:648-660.

• Barker, Cox, Mackie, and Masson. Vacuum Seed Sowing Manifold: a novel device for high-throughput sowing of Arabidopsis seeds (2013). Plant Methods 9, 41.

• Toyota M, Gilroy S (2013) Gravitropism and mechanical signaling in plants. American Journal of Botany 100:111-125.

• Swanson SJ, Gilroy S (2013) Calcium: from root macronutrient to mechanical signal. In The Hidden Half (Beekman T and Eshel A eds) CRC Press, New York, chpt 20:1-13.

• Swanson SJ, Gilroy S (2013) Imaging changes in cytoplasmic calcium using the Yellow Cameleon 3.6 biosensor and confocal microscopy. Methods Molecular Biology 1009:291-302.

• Kim HS, Czymmek KJ, Patel A, Modla S, Nohe A, Duncan R, Gilroy S, Kang S. (2012) Expression of the Cameleon calcium biosensor in fungi reveals distinct Ca(2+) signatures associated with polarized growth, development, and pathogenesis. Fungal Genetics and Biology 49: 589-601.

• Choi, W-G., Swanson, S.J., Gilroy, S. (2012) High resolution imaging of Ca2+, Redox, ROS and pH using GFP biosensors. The Plant Journal, 70, 118-128.