产品名称单层纳米过滤器的培养膜片,Integrated Chips Integration Chips EmailLinkedInPrintTwitter PLUG & PLAY DROPLETS FLOW CONTROLLER ELASTOPLASTIC CHIPS Standard chip Culture Patch tissue Patch
品牌mesobiotech
产品货号单层纳米过滤器的培养膜片,Integrated Chips Integration Chips EmailLinkedInPrintTwitter PLUG & PLAY DROPLETS FLOW CONTROLLER ELASTOPLASTIC CHIPS Standard chip Culture Patch tissue Patch
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联系人李先生
联系电话18618101725
产品说明


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MesoBioTech通过合理设计和标准化制造微流控芯片,自动控制器和配件,专注于集成解决方案。 这些解决方案应适用于各种各样的应用程序,从而损害其简单性,灵活性,多功能性和前景:


  • 通用微流控产品
  • 即插即用的微流处理集成芯片
  • 补丁和filtres可逆组装集成芯片
  • 流量控制器,用于多功能流量开关和调节
  • 用于流连接,切屑夹紧等的附件
  • 基于干细胞的检测产品
  • 单层纳米纤维培养贴片
  • 用于细胞自动处理的培养控制器
  • 使用人多能干细胞的组织贴剂
  • 片上器官进行集成建模
一、INTEGRATION CHIPS


INTEGRATION CHIPS

The integration chips are formed with two plastic plates, each having an elastomeric thin film to ensure good sealing when clamped. They are typically used for patch and filter integration for which micro-chambers and micro-channels are predefined in both plastic plates.

organ_ctc

For filtration, one inlet (outlet) channel is patterned on the upper (lower) plate with a Luer connector. An elastomeric thin film with some removed areas can be added to improved the sealing. Similarly, inlet and outlet chambers are patterned in both plates for organ on-a-chip applications. When a tissue patch is placed between the two plates, culture media containing different factors can flow in the upper and lower chambers separated by the patch so tissue model such as skin, alveolar, stomach, brain-blood are envisaged.

ctcorganchipPhoto

Good sealing is achievable with a loose-leaf clamp or screw enhanced assembling. After fluidic processing with the chip, the integrated elements can be released for other operations. Clamps


二、INTEGRATED CHIPS

Chip with luer connectorsExample of integrated chip with Luer connectors

Integrated chips are manufactured by mass production. The basic element of the chip (upper part) is a plastic plate with standard Luer connectors and an elastomeric thin film for conformal contact with the lower part in which microfluidic circuits can be patterned. While the lower part can be a sheet in plastic, elastomeric, glass, silicon or other materials, standard elements such as mixing Y-channels, gradient Xmas trees, droplet generators, etc. can be patterned.  The upper and the lower part of the chip can be reversely or irreversibly assembled.

With a simple mechanic clamp, good sealing is achievable.  Otherwise,  MesoBioTech offers irreversibly assembled devices for common uses.

NanoInsert     chips4 small

四、FLUIDIC ACCESSORIES

MsoBioTech provides patent protected accessories for general microfluidics and stem cell devices, including

  • Bottle caps: Typically, a metallic female thread and a female Luer are embedded respectively in the center of and aside a bottle cap. Caps with 5 female threads is also available. These female threads are compatible with the commonly used fluidic connectors such as female Luer with barb panel mount and bulkhead, female Luer with male thread, flat-bottom nuts with ferrule, etc.

Cap1b Cap5         IMG_20180124_161457.jpg

  • Chip clamps: Including i) Hand screw open-clamp, ii) Hand screw close-clamp, iii) Inox loose-leaf clamp, and iv) Hand screw multiple open-clamps (patent protected). Good sealing can be achieved with our elastoplastic chips.

Clamp1  clampscrewSmall  Clamp  Clamp4

  • Patch handling device for Boyden chamber like cell based assays with 2 inch dish and 6 well plates.

Boyden device    BoydenB.jpg

  • Luer connectors and tubes: They are selected for various routing work with MesoBioTech chips and instruments.

accesories

六、ORGAN ON-A-CHIP

OrganChipSchematic

Microfluidic device for patch integration

Integration of a tissue patch into a chip gives rise to organ on-a-chip models. Typically, a tissue patch made two types of cells are inserted in the interface of two elastoplastic plates. After clamping, solutions are injected to create physiological conditions. 

One examples is alveolus modeling. By differentiating hiPSCs into epithelial and endothelial cells on upper and lower side of a culture patch, an alveolar tissue is obtained and then integrated into a microfluidic devise to recapitulate the pulmonary physiological conditions. By injecting cell factors and/or nano-materials, it is possible to study the translocation effect at the alveolar epithelium level.

patch system

Organ on-a-chop model with different tissue patches

More generally,  integration of several tissue patches of different cell types into a microfluidic system will allow creating a body-like circulatory or micro-physiological system to investigate the mutual influences of different tissues.  For example, one can co-culture of human intestine, liver, skin and kidney cells with a medium circulation loop for drug testing.

七、TISSUE PATCH

TissuePatch

Tissue patch

Tissue patches are mostly derived from human induced pluripotent stem cells (hiPSCs). By changing the culture parameters, flat and hemisphere colonies are generated in each of the honeycomb compartment. Homogeneous cardiac and neuron tissues are produced, showing up-regulated gene expression of the specific proteins and regular beating or spontaneous firing.

When placed on a multi-electrode array, characteristic drug responses are observed. Tissue patches of co-cultured two cell layers are available, enabling different tissue models. More sophisticated tissue patches are also  obtainable for  three-dimensional tissue modeling.

EBEmbryoid body of hiPSCs

NeuronPatchNeurons and astrocytes on patch


八、CULTURE CONTROLLER

 

? Culture Controller? is a prototype for long-term culture and differentiation of human induced pluripotent stem cells (hiSPCs). It is designed for in-vivo mimicking  of nutrient feeding in a perfusion-diffusion regime.   It allows reducing time and laborious efforts as well as risks of contamination.

btyControl Unit

Typically, a ?Culture Controller? is composed of a central unit to control micro-flow processing, a culture unit placed in an incubator and a cold unit to store and supply culture media with selected factors. The whole hiPSC differentiation processes can then be programmed with minimum human intervention and good reproducibility.

The center unit of the system can also be combined with other fluidic element, allowing automated cell sorting, labeling, SEM sample preparing, etc.

The system is applicable to both dish and chip based assays as well as Culture Patch and Tissue Patch developed by MesoBioTech. More sophisticated culture systems are under exploration.

btyCulture Unit