Mycobacteria Biphasic L-J Culture Medium (Culture Method)/ Reagents kits,encode_specification/price/image

Product Detail

INTENDED USE

This product is intended for mycobacteria isolation and culture.

PRINCIPLE

The product is a new pattern L-J medium used to culture mycobacterium. The solid part consists of all the improved ingredients of L-J medium common to both domestic and foreign professionals; The liquid part consists of basic medium, growth promoting agent, antibacterial agent, and chromogenic agent. Typical colony can form on the slant of the culture medium, as with chromogenic agent, pink or red particles will appears in the liquid part.

COMPONENTS

Solid culture medium, liquid culture medium, sterile diluent, antibacterial agent, package insert

STORAGE AND VALIDITY

· Sealed and stored at 2-8°C in a cool and dry place.

· The validity duration is 6 months

· Use immediately after unsealing.

· Do not freeze.

· Use immediately once antibacterial agent is dissolved, and it can be stored at 2-8°C for 72h and at -20°C 6 months only after dissolution.

SPECIMEN COLLECTION

· Human sputum as specimen for the test, which should be digested through alkaline treatment if contaminated.

· Best result will be likely to obtain with first mouthful of sputum spited out of deep lung in the morning.

· Timely detection is recommended after specimen collection, otherwise store at (2-8°C) in the fridge and finish the test within 72 hours.

· Pretreatment of the specimen: alkali treatment and centrifugation

PROCEDURE

· Take out antibacterial agent, open the cap, and shake up after adding the sterile diluent.

· Suck up 100ul of mixed antibacterial agent, then add into the liquid culture medium and shake up.

· Add the mixed culture medium into the solid culture medium slant.

· Inoculate 0.1ml of already treated specimen onto the slant of the solid culture medium; Do the same thing with the second biphasic L-J medium as the control test.

· Tilt the tube to let the liquid to submerge the entire surface of solid medium, keep the position at 37°C in incubator for 48h, and erect the tube to continue culture.

REFERNCE VALUE

· Positive: Detection on positive control material

· Negative: Detection on negative control material