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96well Responsive Plate
96well Responsive Plate
Brand:
Cosmo Biosciences Inc.
Model:
12 well /96 well plate,35mm dish
Updated:
4/20/2022
  
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  • Product Detail
    Applications
    · Ideal for harvesting fragile cells such as stem cells,neurons etc.
    · Collection of intact and viable single cell suspension.
    · Stem cell culture and tissue engineering culture.
    · Research on extra cellular matrix.
    Feature
    · Made of special polymer Material·
    · Temperature responsive polymer is fixed to the surface of the cultureware at nano-thickness level.
    · Cells are cultured at 37°C and collectable approximately 10-30min after controlling temeperature to 20-25°C.
    Description
    · Collection of intact viable cells without enzymatic processing.
    · Trypsin is well known for its deterioration to the functions of cells. Maintenance of their activities is possible with Cosmo’s
    advanced culture ware is used for cell culture.
    · Harvest cells by mere reduction of temperature.
    · It completely do without trypsin when collecting cells since cell detachs by just the culture surface response.So that the
    Processing would save time 80%.
    · 96 multiwell format is especially suitable for cell cloning by limited dilution.

    Ordering Information

    Cat. No.

    Name

    Description

    2101-035

    35mm Culture Dish

    20/Column

    2101-135

    35mm Culture Dish

    100/Case

    1102-096

    Responses Cell Culture Plate 96well

    10/pack

    1102-196

    Responses Cell Culture Plate 96well

    50/pack


    Instructions Culturing cells
    · First of all, pre-warm the medium to 37°C in the incubator.
    · When seeding cells, please do use the pre-warmed medium to ease attachment of cells.
    · Seed cells evenly over the culture surface to prevent cells being concentrated in one area.
    · The surface becomes hydrophobic above 32°C therefore cells must be cultured above this point.
    · Medium change can be carried out as if using normal culture ware without polymer. Please use pre-warmed medium for change.
    · When cells are difficult to attach, please use medium that contains FCS.
    · Attachment time of the cells may vary depending on the type of the cell.
    Detachment of cells
    · The surface of the cultureware becomes hydrophilic under 32°C. Cells are easier to detach under this temperature.
    · When collecting cells, lower the temperature down to approximately 20°C and wait about 30 minutes. Depending on the type of the cells cultured, they detach automatically.
    · Same results can be achieved by leaving the cultureware in the refrigerator set at 4 degrees celsius for 10 minutes.
    · The cells that did not come off automatically can be detached by agitating them by pipet with medium.
    · Some cells may not detach completely. The results may vary by the type of the cells.
    · Detachment of cells are easier when the temperature is lowered before cells become confluent.
    · The product is intended for one time use only. Please do not re-use the product.
    · The product is only for laboratory use.
    About more advanced responses cell culture ware details , please don't hesitate to contact us:
    E-mail : techservicecosmobrand.com.cn
    Cosmo was founded in 2004, an expert CDMO of sterile and non sterile for life science and medical devices. we own certified class 7 cleanroom and operated by ISO 13485 and GMP regulation.


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