产品标题 产品货号 产品规格 厂家
Transfection Collection™ : SBE Transient Pack (TGFβ/SMAD Signaling Pathway) - The Transfection Collection79272500 reactionsbpsbioscience
Transfection Collection™ : SRE Transient Pack (MAPK/ERK Signaling Pathway) - The Transfection Collection79271500 reactionsbpsbioscience
Transfection Collection™ : TCF/LEF Transient Pack Wnt / β-catenin Signaling Pathway - The Transfection Collection79273500 reactionsbpsbioscience
BCMA / Luciferase - CHO Recombinant Cell Line - Cell Line Rentals797242 vialsbpsbioscience
BCMA / NF-κB – Reporter HEK293 Recombinant Cell Line - Cell Line Rentals797552 vialsbpsbioscience
CD137/NF-κB Reporter - HEK293 Recombinant Cell Line - Cell Line Rentals792892 vialsbpsbioscience
CD137L (4-1BBL) CHO-K1 Recombinant Cell Line - Cell Line Rentals605232 vialsbpsbioscience
CD19 CHO Recombinant Cell Line (Low, Medium and High Expression) - Cell Line Rentals79561-L 79561-M 79561-H2 vials 2 vials 2 vialsbpsbioscience
CD22 CHO Recombinant Cell Line (Medium and High Expression) - Cell Line Rentals79557-M 79557-H2 vials 2 vialsbpsbioscience
CFTR – HEK 293 Recombinant Cell Line - Cell Line Rentals605062 vialsbpsbioscience
CSF1R / SRE – Reporter HEK293 Recombinant Cell Line - Cell Line Rentals79380 60690-1 Size: 10 ml $125 Catalog #: 60690-2 Size: 100 ml $875 Catalog #: 60690-5 Size: 500 mL $3,450 Catalog #: 60690-3 Size: 1 L $6,595 Background: Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bioluminescence. In biology, researchers can take advantage of this reaction and use it as a readout for various biological processes. This has perhaps been exploited most in luciferase reporter cell lines where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase. In this system, transcriptional activation of the gene of interest leads to a level of luciferase expression that is proportional to the level of gene activation. One Step Luciferase Assay System The level of activation is then assessed by lysing the cells and adding the luciferase substrate, D-luciferin. Using the One-Step Luciferase Assay System from BPS Bioscience, this is accomplished in a single step as the lysis buffer has been formulated with D-luciferin. After a brief incubation period, bioluminescence is read on a luminometer. Description: The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: • Sensitive – highly sensitive detection of firefly luciferase activity. • Stable – the signal output is stable for more than two hours, providing flexibility with regard to incubation time • Convenient – simple one-step, homogeneous protocol. • High-throughput – one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications • Compatibility – works well with a variety of common media containing 0-10% serum and phenol red. • Instrumentation – does not require a luminometer with injectors. Synonym(s): Luciferin, Firefly luciferase, luciferase buffer, one step, step, luciferase detection buffer Format: Components for 10 ml Size See datasheet for further information. Instructions for use: See product datasheet for detailed protocol. Storage / Stability: At least 6 months when stored as directed. Upon first thaw, store in aliquots at -20°C. The reagent may be subjected to several freeze/thaw cycles with no effect on functionality, but it is recommended that freeze/thaw cycles be avoided whenever possible. Application(s): • Monitor firefly (Photinus pyralis) luciferase activity in cultured mammalian cells. • High-throughput screening using luciferase reporter cell lines Notes: • The reagent has been validated in a 96-well format. Other formats will require scaling and optimization by the end-user. • Luciferase Reagent Buffer must be at ~ room temperature (20- 25°C) before use. • Luciferase Assay Reagent should be added to cells for at least 5 minutes before measuring luminescence to allow complete cell lysis. • For maximal light intensity, measure samples within 1 hour of reagent addition. • Avoid exposing to excessive heat or light during incubation. • Different cell lines may exhibit variation in lysis ability and/or luminescence signal and may require slight optimization by the end-user. • Luminescence signal is affected by assay conditions. Results should be compared between samples measured using the same cell type and media/serum combinations. • To analyze multiple plates, include a common control sample in each plate and normalize the luminescence of each plate to the control contained in the same plate. • Background luminescence is a characteristic of luminometer performance, therefore, background luminescence must be subtracted from all readings for accuracy. Warning(s): Protect from light. Avoid freeze/thaw cycles. Scientific Category: Cell-Based Assay Kits Product Type: Reagent Data shown is lot-specific. Contact us for specific information on other lots. *Size * Required Fields Qty: 1 ADD TO CART Bulk QuoteContact Us Quality Assurance Product Datasheets 60690-1.pdf 60690-2.pdf 60690-5.pdf 60690-3.pdf Related Products NFAT Reporter (Luc) – Jurkat Recombinant Cell Line ABL (T315I) Assay Kit TIGIT / NFAT Reporter - Jurkat Cell Line PD-1 / NFAT Reporter - Jurkat Recombinant Cell Line BTLA / NFAT - Luciferase Reporter - Jurkat Recombinant Cell Line" target="_blank">ONE-Step™ Luciferase Assay System (BPS Bioscience, #60690)• Luminometer2 Vials 10 ml $125 Catalog #: 60690-2 Size: 100 ml $875 Catalog #: 60690-5 Size: 500 mL $3,450 Catalog #: 60690-3 Size: 1 L $6,595 Background: Luciferase is the general term given to a class of oxidative enzymes that catalyze reactions that give off light, a process known as bioluminescence. In biology, researchers can take advantage of this reaction and use it as a readout for various biological processes. This has perhaps been exploited most in luciferase reporter cell lines where a promoter region from a gene of interest is placed immediately upstream of the coding sequence for luciferase. In this system, transcriptional activation of the gene of interest leads to a level of luciferase expression that is proportional to the level of gene activation. One Step Luciferase Assay System The level of activation is then assessed by lysing the cells and adding the luciferase substrate, D-luciferin. Using the One-Step Luciferase Assay System from BPS Bioscience, this is accomplished in a single step as the lysis buffer has been formulated with D-luciferin. After a brief incubation period, bioluminescence is read on a luminometer. Description: The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: • Sensitive – highly sensitive detection of firefly luciferase activity. • Stable – the signal output is stable for more than two hours, providing flexibility with regard to incubation time • Convenient – simple one-step, homogeneous protocol. • High-throughput – one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications • Compatibility – works well with a variety of common media containing 0-10% serum and phenol red. • Instrumentation – does not require a luminometer with injectors. Synonym(s): Luciferin, Firefly luciferase, luciferase buffer, one step, step, luciferase detection buffer Format: Components for 10 ml Size See datasheet for further information. Instructions for use: See product datasheet for detailed protocol. Storage / Stability: At least 6 months when stored as directed. Upon first thaw, store in aliquots at -20°C. The reagent may be subjected to several freeze/thaw cycles with no effect on functionality, but it is recommended that freeze/thaw cycles be avoided whenever possible. Application(s): • Monitor firefly (Photinus pyralis) luciferase activity in cultured mammalian cells. • High-throughput screening using luciferase reporter cell lines Notes: • The reagent has been validated in a 96-well format. Other formats will require scaling and optimization by the end-user. • Luciferase Reagent Buffer must be at ~ room temperature (20- 25°C) before use. • Luciferase Assay Reagent should be added to cells for at least 5 minutes before measuring luminescence to allow complete cell lysis. • For maximal light intensity, measure samples within 1 hour of reagent addition. • Avoid exposing to excessive heat or light during incubation. • Different cell lines may exhibit variation in lysis ability and/or luminescence signal and may require slight optimization by the end-user. • Luminescence signal is affected by assay conditions. Results should be compared between samples measured using the same cell type and media/serum combinations. • To analyze multiple plates, include a common control sample in each plate and normalize the luminescence of each plate to the control contained in the same plate. • Background luminescence is a characteristic of luminometer performance, therefore, background luminescence must be subtracted from all readings for accuracy. Warning(s): Protect from light. Avoid freeze/thaw cycles. Scientific Category: Cell-Based Assay Kits Product Type: Reagent Data shown is lot-specific. Contact us for specific information on other lots. *Size * Required Fields Qty: 1 ADD TO CART Bulk QuoteContact Us Quality Assurance Product Datasheets 60690-1.pdf 60690-2.pdf 60690-5.pdf 60690-3.pdf Related Products NFAT Reporter (Luc) – Jurkat Recombinant Cell Line ABL (T315I) Assay Kit TIGIT / NFAT Reporter - Jurkat Cell Line PD-1 / NFAT Reporter - Jurkat Recombinant Cell Line BTLA / NFAT - Luciferase Reporter - Jurkat Recombinant Cell Line" target="_blank">ONE-Step™ Luciferase Assay System (BPS Bioscience, #60690)• Luminometerbpsbioscience
Firefly Luciferase - CHO Recombinant Cell Line - Cell Line Rentals797252 Vialsbpsbioscience
Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line - Cell Line Rentals606282 vialsbpsbioscience
GITR / NF-κB Luciferase Reporter - Jurkat Cell Line - Cell Line Rentals605462 vialsbpsbioscience
ICOS/NFAT Reporter-Jurkat Recombinant Cell Line - Cell Line Rentals796682 vialsbpsbioscience
ICSOL - CHO Recombinant Cell Line - BPS Bioscience796352 vialsbpsbioscience
IKCA1 (KCNN4) - HEK293 Recombinant Cell Line - Cell Line Rentals903302 vialsbpsbioscience
LILRB4 NFAT-Luciferase Reporter Jurkat Recombinant Cell Line - Cell Line Rentals797502 vialsbpsbioscience
NaV1.7–HEK293 Recombinant Cell Line - Cell Line Rentals605072 vialsbpsbioscience
Nav1.8/β2 - HEK293 Recombinant Cell Line - Cell Line Rentals605212 vialsbpsbioscience
NET – CHOK1 Recombinant Cell Line - Cell Line Rentals605572 vialsbpsbioscience
NF-κB (GFP) – Reporter HEK293 Recombinant Cell Line - Cell Line Rentals794022 vialsbpsbioscience
OX40 / NF-κB Reporter – HEK293 Recombinant Cell Line - Cell Line Rentals604822 vialsbpsbioscience
PD-1 (Mouse) / NFAT - Reporter - Jurkat Recombinant Cell Line - Cell Line Rentals797622 vialsbpsbioscience
NLRP3 (K232A), His-FLAG-Tag (Sf9-derived)4074110 μgbpsbioscience
EGFR (T790M, C797S, L858R), His-Tag, GST-tag4035110 μgbpsbioscience
Mouse EGFR, FLAG-tag4019510 µgbpsbioscience
EGFR (T790M), His-tag, GST-tag4018810 µgbpsbioscience
EGFR (L858R), GST-tag4018910 µgbpsbioscience
Western ECL Substrate79572-1 79572-2 79572-3200 ml 500 ml 1 Lbpsbioscience
NeutrAvidin Plates79512-1 79512-21 plate 5 platesbpsbioscience
MS-275 (Entinostat)2701125 mgbpsbioscience
Geldanamycin2700810 µlbpsbioscience
Transfection Collection™ : NF-κB Transient Pack ( NF-κB Signaling Pathway)79268500 reactionsbpsbioscience
PD-L2 (CD273), Fc fusion (Human) HiP™71107100 µgbpsbioscience
PD-1[Biotinylated]:PD-L2 Inhibitor Screening Assay Kit7200696 reactionsbpsbioscience
PD-L2 (CD273), Fc fusion, Biotin-labeled (Human)7110850 µgbpsbioscience
Immuno Buffer 17931150 mlbpsbioscience
PD-1:PD-L2[Biotinylated] Inhibitor Screening Assay Kit7200496 reactionsbpsbioscience
Transfection Collection™ : SBE Transient Pack (TGFβ/SMAD Signaling Pathway)79272500 reactionsbpsbioscience
Thaw Medium 160187-1 60187-2100 ml 500 mlbpsbioscience
Growth Medium 1K79533500 mlbpsbioscience
Growth Medium 1C79532500 mlbpsbioscience
Growth Medium 1B79531500 mlbpsbioscience
MLK2, GST-tag4110310 µgbpsbioscience
MLK3, GST-tag4110410 µgbpsbioscience
Notch Signaling Pathway Notch1/CSL Reporter - HEK293 Recombinant Cell Line606522 vialsbpsbioscience
MLK4, GST-tag4110510 µgbpsbioscience
c-Myc, His-tag40453100 µgbpsbioscience
Streptavidin-HRP7974215 µlbpsbioscience
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