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| Product Name | Vitamin B1 (Thiamin) HPLC Assay |
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| Description | For the determination of vitamin B1 a precipitation step to remove high molecular substances is performed first. After centrifugation the supernatant is mixed with a derivatisation solution and incubated for 10 min at 60°C. The fluorescent probe is then cooled (2-8°C), centrifuged and injected into the HPLC system. The isocratic separation via HPLC at 30°C lasts 10 minutes. The chromatograms are recorded by a fluorescence detector. The quantification is performed with the delivered EDTA-blood calibrator; the concentration is calculated via integration of the peak heights respectively areas. Sample Types: EDTA whole blood. Time: 20 minutes • The Eagle Biosciences Vitamin B1 (Thiamin) HPLC Assay makes it possible to determine the vitamin in an easy, fast and precise method. The kit includes all reagents in ready to use form for preparation and separation of the samples with exception of the columns (IC2201rp) and the controls (IC2201ko). Thiamin is found free and as mono-, di- and triphosphate. The different forms can be transferred into each other. The metabolic active form is thiamin diphosphate in the body and thiamin triphosphate in the brain. Thiamin diphosphate (TDP) works as thermostable coenzyme in many enzymes of the carbohydrate and amino acid metabolism. In most reactions TDP catalyzes the cleavage of C-C bonds. In the nervous system vitamin B1 is needed for the stimulation of nerve cells. |
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| Size | 100 tests |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Vitamin B1, Thiamin, Thiamine, TDP, Thiamin diphosphate |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | VB131-H100 |
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| Order / More Info | Vitamin B1 (Thiamin) HPLC Assay from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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