| 产品详情 |
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| Product Name | Cyclic GMP EIA Kit |
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| Description | The Cyclic GMP (cGMP) EIA kit is designed to quantitatively measure cGMP present in lysed cells, EDTA plasma, urine, saliva and tissue culture media samples. For samples where the levels of cGMP are expected to be relatively high, the regular format for the assay can be used. For samples with expected low levels of cGMP, an optional acetylation protocol can be used. The kit is unique in that all samples and standards are diluted into an acidic Sample Diluent, which contains special additives and stabilizers, for cGMP measurement. This allows plasma, urine and saliva samples to be read in an identical manner to lysed cells. Acidified samples of cGMP are stable and endogenous phosphodiesterases are inactivated in the Sample Diluent. A cGMP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. A clear microtiter plate coated with an antibody to capture mouse IgG is provided and a neutralizing Plate Primer solution is added to all the used wells. Standards or diluted samples, either with or without acetylation, are pipetted into the primed wells. A cGMP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a mouse monoclonal antibody to cGMP to each well. After a 2 hour incubation, the plate is washed and substrate is added. The substrate reacts with the bound cGMP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the cGMP in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers. Direct EIA (Enzyme Immunoassay). Intra Assay Precision (Regular): Three human urine samples were diluted with Sample Diluent and run in replicates of 20 in an assay. The mean and precision of the calculated cGMP concentrations were: Sample 1- 8.5 pmol/mL, 6.3% CV Sample 2- 4.0 pmol/mL, 7.8% CV Sample 3- 1.2 pmol/mL, 13.6% CV Intra Assay Precision (Acetylated): Three human urine samples were diluted with Sample Diluent, acetylated and run in replicates of 20 in an assay. The mean and precision of the calculated cGMP concentrations were: Sample 1- 8.1 pmol/mL, 3.8% CV Sample 2- 3.8 pmol/mL, 5.2% CV Sample 3- 1.2 pmol/mL, 8.2% CV Inter Assay Precision (Regular): Three human urine samples were diluted with Sample Diluent and run in duplicates in twelve assays run over multiple days by three operators. The mean and precision of the calculated cGMP concentrations were: Sample 1- 8.5 pmol/mL, 6.5% CV Sample 2- 4.3 pmol/mL, 7.0% CV Sample 3- 1.5pmol/mL, 8.3% CV Inter Assay Precision (Acetylated): Three human urine sample weres diluted with Sample Diluent, acetylated and run in duplicates in twelve assays run over multiple days by three operators. The mean and precision of the calculated cGMP concentrations were: Sample 1- 8.7 pmol/mL, 12.3% CV Sample 2- 4.3 pmol/mL, 14.3% CV Sample 3- 1.5 pmol/mL, 14.2% CV. Range: Regular: 0.5 - 32 pmol/ml. Acetylated: 0.3125 - 20 pmol/ml. Sensitivity: 0.28 pmol/ml. |
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| Size | 96 well |
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| Concentration | n/a |
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| Applications | EIA kit used to quantitatively measure cGMP present in samples. RUO |
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| Other Names | StressXpress® cGMP; 3',5'-cyclic GMP EIA Kit, Guanosine cyclic monophosphate EIA Kit, Cyclic 3',5'-GMP; Guanosine 3',5'-cyclic phosphate EIA Kit |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | SKT-211-96 |
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| Order / More Info | Cyclic GMP EIA Kit from STRESSMARQ BIOSCIENCES INC. |
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| Product Specific References | n/a |
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