| 产品名称 | VeriScript Reverse TranscriptaseVeriScript Reverse Transcriptase is available 1 time from Biovision labs |
| 产品货号 | 9224-1000 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biovision labs |
| 产品概述 | |
| 产品详情 | VeriScript Reverse TranscriptaseVeriScript Reverse Transcriptase is available 1 time from Biovision labs9224-1000 | VeriScript Reverse Transcriptase size: 1000 UN |MeSH DataName
Reverse Transcriptase Polymerase Chain Reaction
Tree numbers
Product images Other name
Verscript
Aa sequence
N/A
Unit
One unit is the amount of enzyme activity that incorporates 1 nmole of dTTP into acid insoluble material in 10 minutes at 37°C using poly(rA)-oligo(dT)18 as template-primer.
Reesults
N/A
Molecular weight
N/A
Source
Recombinant
Storage condition
-20°C
Level of endotoxin
N/A
Binding ability
N/A
Tested applications
SDS-PAGE, N/A
Notes
For Research Use Only! Not to be used in humans
Recombinant
Yes
Tested activity
200 unit/μl
Purification
≥95%
Background
VeriScript Reverse Transcriptase is an engineered version of M-MLV Reverse Transcriptase with reduced RNase H activity and increased thermostability, allowing for first-strand cDNA synthesis with more full length cDNAs up to 12.5 kb. This DNA polymerase can synthesize complementary DNA from single-stranded DNA, RNA, or a RNA:DNA hybrid.
Before use
Centrifuge the vial prior to opening.
Shipping under
Gel pack
Concentration
N/A
Physical properties
Recombinant VeriScript Reverse Transcriptase in 20mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01% Igepal CA-630, and 50% Glycerol.
Biological activity
N/A
Physical appearance
Liquid
Synonym
Verscript
How to reconstitute
N/A
Description
Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
Goup
reverse transcription
Gene targetVeriScript
Transcriptase
Short name
VeriScript Reverse Transcriptase
Technique
reverse
Alternative name
VeriScript Reverse Transcriptase
Alternative technique
transctription
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