| 产品名称 | Human Mouse Rat UDP Glucose Ceramide Glucosyltransferase UGCG ELISA Kit 96 well plateHuman Mouse Rat UDP Glucose Ceramide Glucosyltransferase UGCG ELISA Kit 96 well plate is available 3 times from Bioma labs |
| 产品货号 | EKU09079 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Bioma labs |
| 产品概述 | |
| 产品详情 | Human Mouse Rat UDP Glucose Ceramide Glucosyltransferase UGCG ELISA Kit 96 well plateHuman Mouse Rat UDP Glucose Ceramide Glucosyltransferase UGCG ELISA Kit 96 well plate is available 3 times from Bioma labsEKU09079 | Human/Mouse/Rat UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit, 96 well plate size: 96T | 831.00 EUR EKU09079 | Human/Mouse/Rat UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit, 96 well plate size: 5x96T |1. Gene infoIdentity12524 GeneUGCG Long gene nameUDP-glucose ceramide glucosyltransferase Synonyms GenBank acession Locus9q31.3 Discovery year1997-08-28 RefSeq identity Havana BLAST/BLATOTTHUMG00000020498 2. Gene infoIdentity20460 GeneGBGT1 Synonyms GenBank acession Locus9q34.2 Discovery year2004-05-17 RefSeq identity Havana BLAST/BLATOTTHUMG00000020853 Locus Specific Databases MeSH DataTree numbers
Product images   EKU09079 | Human/Mouse/Rat UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit, 96 well plate size: 10x96T | 6,376.00 EURVerified reactivity
Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)
Detection limits
1.56-100 ng/mL
Sensitivity limit
0,53 ng/mL
Samples to be analyzed
Tissue homogenates, cell lysates and other biological fluids
Assay duration
3 hours
Verified applications
ELISA
Assay class
Sandwich
ELISA detection
Colorimetric
Protein number
Please refer to Uniprot
Alternate protein number
Please refer to SwissProt
Gene number
Please refer to GenBank
Gene name
UDP Glucose Ceramide Glucosyltransferase
Alternate gene name
GCS; GLCT-1; Glucosylceramide Synthase; N-acylsphingosine D-glucosyltransferase
Estimated production time
7-11 business days
Shipping requirements
Blue ice
Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Use for
8 months
ELISA's specificity
This assay has high sensitivity and excellent specificity for detection of UDP Glucose Ceramide Glucosyltransferase (UGCG). No significant cross-reactivity or interference between UDP Glucose Ceramide Glucosyltransferase (UGCG) and analogues was observed.
ELISA's cross-reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Assay principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP Glucose Ceramide Glucosyltransferase (UGCG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to UDP Glucose Ceramide Glucosyltransferase (UGCG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP Glucose Ceramide Glucosyltransferase (UGCG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UDP Glucose Ceramide Glucosyltransferase (UGCG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Protocol
Please see ELISA's datasheet, otherwise contact us
Precision of the test
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UDP Glucose Ceramide Glucosyltransferase (UGCG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UDP Glucose Ceramide Glucosyltransferase (UGCG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
ELISA's stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
QC
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research main area
Enzyme & Kinase;Metabolic pathway;Endocrinology;
Warnings
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Notes
For research use only. Not for diagnostic procedures.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name
Mus musculus,Rattus norvegicus
Description
A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Gene targetUDP
Glucose
Ceramide
Glucosyltransferase
UGCG
Short name
/Mouse/ UDP Glucose Ceramide Glucosyltransferase (UGCG) ELISA Kit, 96
Technique
ELISA kit, ELISA, Mouse
Host
mouse
Species
Rat
Alternative name
H. sapiens/Mouse/Rat UDP Glucose Ceramide Glucosyltransferase (UGCG) Enzyme-linked immunosorbent assay reagent, 96 well plate
Alternative technique
elisas
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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