| 产品名称 | Human Semaphorin 7A SEMA7A ELISA Kit 96 well plateHuman Semaphorin 7A SEMA7A ELISA Kit 96 well plate is available 3 times from Bioma labs |
| 产品货号 | EKU07269 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Bioma labs |
| 产品概述 | |
| 产品详情 | Human Semaphorin 7A SEMA7A ELISA Kit 96 well plateHuman Semaphorin 7A SEMA7A ELISA Kit 96 well plate is available 3 times from Bioma labsEKU07269 | Human Semaphorin 7A (SEMA7A) ELISA Kit, 96 well plate size: 96T | 809.00 EUR EKU07269 | Human Semaphorin 7A (SEMA7A) ELISA Kit, 96 well plate size: 5x96T |MeSH DataTree numbers
Product images EKU07269 | Human Semaphorin 7A (SEMA7A) ELISA Kit, 96 well plate size: 10x96T | 6,202.00 EURVerified reactivity
Homo sapiens (Human)
Detection limits
0.156-10 ng/mL
Sensitivity limit
0,057 ng/mL
Samples to be analyzed
Serum, plasma, tissue homogenates and other biological fluids
Assay duration
3 hours
Verified applications
ELISA
Assay class
Sandwich
ELISA detection
Colorimetric
Protein number
O75326
Alternate protein number
Please refer to SwissProt
Gene number
8482
Gene name
Semaphorin 7A
Alternate gene name
CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; SEMAK1; SEMAL; Semaphorin 7A,GPI Membrane Anchor; John Milton Hagen Blood Group; Semaphorin-K1; Semaphorin-L
Estimated production time
7-11 business days
Shipping requirements
Blue ice
Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Use for
8 months
ELISA's specificity
This assay has high sensitivity and excellent specificity for detection of Semaphorin 7A (SEMA7A). No significant cross-reactivity or interference between Semaphorin 7A (SEMA7A) and analogues was observed.
ELISA's cross-reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Assay principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin 7A (SEMA7A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Semaphorin 7A (SEMA7A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Semaphorin 7A (SEMA7A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Semaphorin 7A (SEMA7A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Protocol
Please see ELISA's datasheet, otherwise contact us
Precision of the test
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 7A (SEMA7A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 7A (SEMA7A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
ELISA's stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
QC
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research main area
Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Autoimmunity;
Warnings
The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Notes
For research use only. Not for diagnostic procedures.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description
A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals.
A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Gene targetSemaphorin
SEMA7A
Short name
Semaphorin 7A (SEMA7A) ELISA Kit, 96
Technique
ELISA kit, ELISA
Host
antigen assay ELISA kit 1 Kit (96 Wells)
Species
Human
Alternative name
H. sapiens Semaphorin 7A (semaphorin 7A, glucose-6-phosphate isomerase membrane anchor (John Milton Hagen blood family)) Enzyme-linked immunosorbent assay reagent, 96 well plate
Alternative technique
elisas
Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832, semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group), CD108 and CDw108 and H-SEMA-K1 and H-Sema-L and JMH and SEMAK1 and SEMAL, SEMA7A and IDBG-22070 and ENSG00000138623 and 8482, protein binding, Cell surfaces, Sema7a and IDBG-171171 and ENSMUSG00000038264 and 20361, SEMA7A and IDBG-631814 and ENSBTAG00000011446 and 614707
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