产品详情 |
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Antigenic Specificity | ARNT/HIF-1 beta |
Clone | polyclonal |
Host Species | Rabbit |
Reactive Species | human, mouse, rat, bovine, fish, ferret, sheep |
Isotype | n/a |
Format | unpurified |
Size | 0.025 ml |
Concentration | n/a |
Applications | Western Blot, Simple Western, Chromatin Immunoprecipitation, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Gel Supershift Assay, Chromatin Immunoprecipitation (ChIP). This HIF-1 beta antibody is useful for Western blot, Immunoprecipitation, Chromatin Immunoprecipitation and Immunohistochemistry on paraffin-embedded sections. This antibody did not work in Immunocytochemistry. By Western blot, this antibody recognizes a band at 92 kDa representing HIF-1 beta in induced tissues and cells. Background non-specific bands are minimal on MCF7 human nuclear extracts. Background may increase with whole cell extract preparations. Lower dilutions may be needed for whole cell extract preps. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 17003483). Use in Gel super shift assay reported in scientific literature (PMID: 11046109). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
Description | The ARNT/HIF-1 beta Antibody from Novus Biologicals is a rabbit polyclonal antibody to ARNT/HIF-1 beta. This antibody reacts with human, mouse, rat, bovine, fish, ferret, sheep. The ARNT/HIF-1 beta Antibody has been validated for the following applications: Western Blot, Simple Western, Chromatin Immunoprecipitation, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Gel Supershift Assay, Chromatin Immunoprecipitation (ChIP). |
Immunogen | Fusion protein to human HIF-1 beta containing amino acids 496-789. [UniProt# P27540] |
Other Names | ARNT protein, aryl hydrocarbon receptor nuclear translocator, BHLHE2, HIF-1 Beta, HIF1B, HIF-1-beta, HIF1-beta, HIF1BETA, hypoxia-inducible factor 1, beta subunit, Hypoxia-inducible factor 1-beta, nuclear translocator |
Gene, Accession # | ARNT, Gene ID: 405, Accession: P27540, SwissProt: P27540 |
Catalog # | NB100-110SS |
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Order / More Info | ARNT/HIF-1 beta Antibody from NOVUS BIOLOGICALS, LLC |
Product Specific References | PubMed: 11046109, 11093754, 12193413, 12840067, 14668208, 14766942, 16585189, 17003483, 17189520, 18845636, 19295143, 20624928, 21199896, 21447827, 21571835, 21666223, 21685248, 21788340, 22402494, 22406644, 22903824, 23012449, 23604114, 23897427, 24353279, 24777823, 24999590, 25017895, 25872882, 26262842, 27506891, 27565965, 27774982, 28715484, 28963769, 29059365, 29416063, 29875134, 30334616, 30429208 |
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