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| Product Name | Long [R3]IGF-I, recombinant (Media Grade) |
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| Description | Product Specifications:The Product: Long [R3] insulin-like growth factor-I (Long[R3]IGF-I) is an 83 amino acid analog of human IGF-I comprising the complete human IGF-I sequence with the substitution of an Arg (R) for the Glu (E) at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus . Scientists have engineered this analog with the express purpose of increasing the biological activity of the IGF-I molecule. Long [R3]IGF-I is significantly more potent than IGF-I in vitro (Francis, et al. J. Mol. Endocrinol. 8: 213, 1992) and in vivo (Tomas, et al., J. Endocrinol. 137: 413, 1993). The enhanced potency is due to decreased binding of Long [R3]IGF-I to all known IGF binding proteins. These binding proteins normally inhibit the biological actions of IGFs.Scientists have produced Media Grade Long [R3]IGF-I to provide an inexpensive, yet-high quality potent IGF-I analog for use as a growth factor supplement for serum-free or reduced-serum culture media.Source: Long [R3]IGF-I is synthesized recombinantly in E. coli using a patented expression system. The product is harvested from cells as inclusion bodies which are dissolved, desalted, and then correctly folded under oxidising conditions. Chromatography is employed to yield Media Grade Long[R3]IGF-I.Purity: > 95% (by N-terminal sequence analysis)Molecular Weight: 9111.6 daltons and minor peak of 9130 daltons (N-terminal formylated form) confirmed by Mass SpectrometryN-terminal sequence analysis of recombinant product: 18 residues >95% single sequenceBiological Activity: (stimulation of protein synthesis in rat L6 myoblasts) ED50 <10 ng/mlEndotoxin (LAL): <0.1 EU/ugStability: At least 2 years at 2-4°C(lyophilised)Appearance: White PowderState: Dried from 0.1 M acetic acid: under dry nitrogen @ slight vacuum (-25kPa)Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g. <1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles. This is a Gropep specification. Long[R3]IGF-I is a trademark of GroPep. ibt is authorized distributor of GroPep. |
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| Size | 200 µg |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | LongTMR3 Insulin-like Growth Factor-I, Media Grade, recombinant |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | AU200 AM001 AM005 |
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| Order / More Info | Long [R3]IGF-I, recombinant (Media Grade) from IMMUNOLOGICAL & BIOCHEMICAL TESTSYSTEMS GmbH |
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| Product Specific References | n/a |
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