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| Product Name | MMAF Antibody Drug Conjugate (ADC) ELISA |
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| Description | Assay calibrators (antibody MMAF conjugate) and test serum samples are added directly to wells of a microtiter plate that is coated with specific anti-MMAF antibody. Subsequently, a horseradish peroxidase (HRP) conjugated MMAF is added to each well. During the incubation period, the antibody MMAF conjugate competes with the HRP conjugated MMAF for the limited binding sites of anti-MMAF antibody. An immune complex of well coated ``anti-MMAF antibody - HRP conjugated MMAF'' is formed. The unbound antibodies and buffer matrix are removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction, which is terminated with an acidic reagent (i.e. ELISA stop solution). The absorbance is then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the wall of each microtiter well is inversely proportional to the amount of antibody-MMAF conjugate in the test sample. A calibration curve is generated by plotting the absorbance versus the respective antibody-MMAF conjugate concentration for each calibrator on a 4-parameter or log-logit curve fitting. The concentration of antibody-MMAF conjugate in test samples is determined directly from this calibration curve. Species Reactivity: human, mouse, rat, primate. Sample Types: serum, plasma, cell culture. Range: 0.016 - 2.0 µg/mL. Sensitivity: 0.019 µg/mL. Time: 2.5 hours • The Eagle Biosciences MMAF Antibody Drug Conjugate (ADC) ELISA Assay kit is intended for use in the quantitative determination of antibody-MMAF-conjugate level in test sample. It is useful for pre-clinical and clinical pharmacology study of MMAF Antibody Drug Conjugate (ADC). |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | MMAF, Monomethyl auristatin F |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | KTR-758 |
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| Order / More Info | MMAF Antibody Drug Conjugate (ADC) ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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