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| Product Name | ASCA IgG ELISA |
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| Description | The Eagle Biosciences ASCA IgG ELISA Assay Kit is an enzyme immunoassay for the qualitative and quantitative determination of IgG antibodies to Saccharomyces cerevisiae in human serum. Autoantibodies of the diluted patient samples, calibrators, and the control react with mannan (cell surface component of baker's yeast) immobilized on the solid phase of a microtiter plate. ASCA IgG guarantees the specific binding of anti-Saccharomyces cerevisiae IgG antibodies of the specimen under investigation by employing purified mannan of Saccharomyces cerevisiae for coating. Following an incubation period of 60 min at room temperature, unbound sample components are removed by a wash step. The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish perox-idase (HRP). Within the incubation period of 30 min at RT, excessive conjugate is separated from the solid-phase immune complexes by the follow-ing wash step. HRP converts the colorless substrate solution of 3,3',5,5'-tetramethyl benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the antibody concentrations of the calibrators (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve. Evaluating the test by a semi-quantitative method is also possible. Sample Types: serum, plasma. Range: 1-300 U/mL. Sensitivity: 1 U/mL. Time: 2.5 hours • Non-specific inflammatory bowel diseases includ-ing Crohn's disease (Enteritis regionalis) and ul-cerative colitis (UC) are characterized by unknown etiology as well as chronic-remitting inflammatory processes of the intestine. Whereas the inflamma-tion of ulcerative colitis is restricted to the mucosa and submucosa of colon and rectum, Crohn's dis-ease (CD) shows a wide spread inflammation of the gastro-intestinal tract with granuloma for-mation. The risk developing one of these diseases is strongly influenced by immunologic, genetic, in-fectious and environmental factors. The differential diagnosis of inflammatory bowel diseases to chronic diarrhea, recurrent abdominal dolor, infectious colitis, anorexia as well as the differentiation of CD to ulcerative colitis is still a high challenge. The determination of IgA and IgG antibodies to Saccharomyces cerevisiae (baker's yeast) has been described as one important serological marker for the differential diagnosis of Crohn's disease re-cently. Up to 70 % of patients with CD show anti-body levels to Saccharomyces cerevisiae. Although the cause for their occurrence has been unclear, antibodies to Saccharomyces cerevisiae (ASCA) are strongly associated with inflammatory pro-cesses of the intestine. In combination with the detection of autoanti-bodies to atypical anti-neutrophil cytoplasmic Antigenss (aANCA) which are mainly found in patients with ulcerative colitis, ASCA are a valid pa-rameter for the differentiation of Crohn's disease and ulcerative colitis. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | ASSAY EIA ELISA IMMUNOASSAY KIT ASCA TEST IgG, Crohn's, colitis, Saccharomyces cerevisiae ASG31-K01 |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | ASG31-K01 |
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| Order / More Info | ASCA IgG ELISA from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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