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| Product Name | Soluble Semaphorin 4D ELISA Assay Kit |
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| Description | This kit is a sandwich enzyme immunoassay for the direct determination of soluble Semaphorin 4D in human plasma samples. In a first step, STD/sample/CTRL are pipetted into the wells of the microtiter strips, which are precoated with anti Semaphorin 4D antibody. Semaphorin 4D present in the STD/sample/CTRL binds to the pre-coated antibody in the well. In the washing step all non-specific unbound material is removed. In a next step, the conjugate (bivalent Fab bacterial alkaline phosphatase fusion antibody-HRP) is pipetted into the wells and reacts with the soluble Semaphorin 4D forming a sandwich. After another washing step, the substrate (TMB Tetramethylbenzidine) is pipetted into the wells. The enzyme catalysed colour change of the substrate is directly proportional to the amount of Semaphorin 4D. This colour change is detectable with a standard microtiter plate ELISA reader. A dose response curve of the absorbance (optical density, OD at 450 nm) versus standard concentration is generated, using the values obtained from the standards. The concentration of Semaphorin 4D in the sample is determined directly from the dose response curve. Sample Types: EDTA plasma, heparin plasma, and citrate plasma. Range: 62.5 to 2000 pmol/l. Sensitivity: LOD: (0 pmol/l + 3 SD): 12 pmol/l; LLOQ: 31 pmol/l. Time: 4 hours • Semaphorin 4D (SEMA4D or CD100) is a member of a family of transmembrane and secreted proteins that regulates key cellular functions and is involved in cell-cell communication (1-3). Most of the effects of SEMA4D is mediated by plexins (4, 5). SEMA4D participates in numerous physiological processes such as axon guidance, immune regulation, angiogenesis, tumor progression, and bone metabolism (6-9). Cleavage of SEMA4D near the cell membrane through matrix metalloproteinases leads to the biologically active soluble SEMA4D with a molecular weight of 120 kD consisting of 713 amino acids (3, 5, 10). SEMA4D has emerged to a novel therapeutic target in cancer and in bone diseases (11, 12). Semaphorin 4D is widely studied for its role in neural connectivity, vascularization, cell migration, the immune response, tumor progression, and bone remodeling. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Bioactive Sclerostin |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | BI-20405 |
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| Order / More Info | Soluble Semaphorin 4D ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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