| 产品详情 |
| Edit |   |
|---|
| Product Name | Mouse / Rat Active GLP-1 ELISA |
|---|
| Description | The Eagle Biosciences Mouse/Rat Active GLP-1 ELISA Assay Kit is designed, developed and produced for the quantitative measurement of bioactive GLP-1 (7-36) in rodent plasma that usually have limited amount of sample available for analysis. The assay utilizes the two-site ``sandwich'' technique with two selected GLP-1 (7-36) specific antibodies. Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 (7-36) specific antibody and a horseradish peroxidase (HRP) conjugated GLP-1 (7-36) specific antibody is added to each well. After the first incubation period, a ``sandwich'' immunocomplex of ``Streptavidin - Biotin-Antibody - GLP-1(7-36) - HRP conjugated antibody'' is formed and attached to the wall of the plate. The unbound HRP conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36) on the wall of the microtiter well is directly proportional to the amount of GLP-1 (7-36) in the sample. Sample Types: plasma. Range:.85 - 146 pM. Sensitivity: 0.1 pM. Time: 24.5 hours • The Eagle Biosciences Mouse/Rat Active GLP-1 ELISA Assay Kit (enzyme-linked immunoassay kit) is intended quantitative determination of bioactive glucagon-like peptide-1 (7-36) amide [GLP-1 (7-36)] level in rat and mouse plasma samples with only 20 ul of sample volume. E The primary amino acid sequence of GLP-1 peptide is identical among mammalian species, i.e. rat, mouse, pig, human, etc. Eagle Biosciences Mouse/Rat Active GLP-1 ELISA Assay Kit is for research use only and not to be used in diagnostic procedures. |
|---|
| Size | 1 x 96 well |
|---|
| Concentration | n/a |
|---|
| Applications | RUO |
|---|
| Other Names | GLP-1, glucagon-like-peptide-1 |
|---|
| Gene, Accession, CAS # | n/a |
|---|
| Catalog # | GP121-K01 |
|---|
| Price | |
|---|
| Order / More Info | Mouse / Rat Active GLP-1 ELISA from EAGLE BIOSCIENCES INC. |
|---|
| Product Specific References | n/a |
|---|
| 产品资料 |
|
|