| 产品详情 |
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| Product Name | Mouse IgE ELISA KIT |
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| Description | The Eagle Biosciences Mouse IgE ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IgE has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps any IgE present is bound by the immobilized antibody and the detection antibody specific for IgE is binds to the combination of capture antibody- IgE in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IgE present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IgE standard dilutions and IgE sample concentration determined. Sample Types: serum, plasma, cell culture. Range: 312.5 - 10,000 pg/ml. Sensitivity: 156 pg/ml. Time: 3.0 hours • There are five classes of mammalian immunoglobulins: IgA, IgD, IgE, IgG, and IgM. In mice, the IgG class is further divided into four subclasses: IgG1, IgG2a/ IgG2c (strain specific), IgG2b, and IgG3.The general immunoglobulin structure is described as two heavy and two light chain (H2L2) polypeptides linked together by disulfide bridges of cysteine residues. Mouse IgE, also known as reaginic antibody, exists in serum as a 4-chain monomer of 185-200 kDa. Like IgM, the IgE monomers contain a fourth constant region domain. Serum concentrations of IgE are reported to be >10 ug/ml. IgE is cyolytic for mast cells, where it binds to the FcRI receptor and is able to mediate the response of granular and lipid mediators of inflammation, including the PCA reaction. The IgE class switch is induced by IL-4 as part of the Th2 response. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Immunoglobulin G, IgG |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | IGE11-K01 |
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| Order / More Info | Mouse IgE ELISA KIT from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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