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| Product Name | Creatinine Assay Kit (urine normalizing assay) |
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| Description | The Creatinine Microplate Assay is a colorimetric assay for the quantitative analysis of creatinine levels in urine. Urinary creatinine reacts with picric acid under alkaline conditions to produce an orange color, which can be quantified by absorption spectroscopy near the 500 nm wavelength. This reaction, known as the Jaffe reaction, also occurs non-specifically with other components in biological fluids. However, the specific color produced with creatine in this reaction is known to degrade rapidly under acidic conditions (Slot et al.). Heinegard and Tiderstrom showed that the difference in color intensity determined before and after the addition of acid is a direct estimate of creatinine concentration in the sample. Sample Types: urine. Range: 1.0 - 10 mg/dL. Sensitivity: 1.0 mg/dl. Time: 15 min • Creatine (Cr) is produced in the kidney, liver and pancreas, phosphorylated, and transported to the brain and muscle tissue. However, a small proportion of free Cr is converted irreversibly to creatinine (Crn) in the muscular tissue in proportion to the muscle mass. The amount of Crn excreted daily by an individual is relatively constant. Thus, urinary creatinine levels may be used as an index of standardization. 24-h urinary Crn excretion is used to estimate total muscle mass because the rate of non-enzymatic production of Crn from Cr is nearly constant and >90% of the total body Cr is found in muscle tissue. Normal urinary creatinine values for men and women range from 9.7 - 24.7 and 7.9 - 14.2 mmol/24h respectively. Changes in excretion rate may be indicative of impaired renal metabolism. |
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| Size | 1 x 96 well |
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| Concentration | n/a |
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| Applications | RUO |
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| Other Names | Creatinine, Creatine, urine, Crn |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | CRE34-K01 |
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| Order / More Info | Creatinine Assay Kit (urine normalizing assay) from EAGLE BIOSCIENCES INC. |
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| Product Specific References | n/a |
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