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| Product Name | Oct4 Transcription Factor Assay Kit |
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| Description | Oct4 is an essential regulator of embryonic stem cell pluripotency and used as a marker for undifferentiated cells. It also plays a role in gene expression, DNA repair and/or replication, signal transduction and tumorigenesis. Cayman's Oct4 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor-DNA binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the Oct4 response element is immobilized onto the wells of a 96-well plate. Oct4, contained in a nuclear extract, binds specifically to the Oct4 response element. Oct4 is detected by the addition of a specific primary antibody directed against Oct4. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. |
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| Size | 96 well |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | kits assays measures measuring measurements tanscriptions factors TFs Octs four 4 Oct-4 POU5F1 genes targets stems cells research non-radioactive non radioactive sensitive methods detecting detections detects factor-DNA dsDNA deoxyribonucleic acids binds binding activities nuclear extracts enzymes linked enzyme-linked links linkages immunosorbents ELISAs ds double stranded double-stranded sequences response responding elements conjugated HRPs HRP-conjugates conjugates colorimetric cancers biology |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 601080-96WELLS |
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| Price | |
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| Order / More Info | Oct4 Transcription Factor Assay Kit from CAYMAN CHEMICAL COMPANY |
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| Product Specific References | n/a |
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