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| Product Name | ROS Detection Cell-Based Assay Kit (DHE) |
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| Description | Dihydroethidium (hydroethidine or DHE) is a widely used ethidium-based, redox-sensitive fluorescent probe. DHE has been shown to be oxidized by superoxide to form 2-hydroxyethidium (2-OH-E+) (ex 500-530 nm/em 590-620 nm) or by non-specific oxidation by other sources of ROS to form ethidium (E+) (ex 480 nm/em 576 nm).{32281,32282} Given their narrow spectral range, distinguishing between the two species using filter-based optical systems is often difficult and can lead to misreporting of the species of ROS being generated. This assay kit uses DHE as a fluorescent probe for the detection of ROS generation. Antimycin A, an inhibitor of complex III of the mitochondrial electron transport chain, is included as a positive control for ROS generation. N-acetyl Cysteine is included as an antioxidant control. |
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| Size | 96 well |
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| Concentration | n/a |
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| Applications | Cell-Based Assay, Flow Cytometry, High-Throughput Screening |
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| Other Names | Reactive Oxidation Species CBA Kit (dihydroethidium) |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 601290-96WELLS |
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| Order / More Info | ROS Detection Cell-Based Assay Kit (DHE) from CAYMAN CHEMICAL COMPANY |
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| Product Specific References | n/a |
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