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| Product Name | NETosis Assay Kit |
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| Description | Cayman's NETosis Assay Kit provides a simple and fast method for studying the process of NETosis ex vivo. Notably, Cayman's NETosis Assay Kit does not depend upon the DNA component of neutrophil extracellular traps (NETs), as DNA release can occur independently of NETosis. In this kit, primary neutrophils are stimulated to release NETs with either PMA or a calcium ionophore (both included). Unbound neutrophil elastase is washed away following NET generation. Following digest of NET DNA by S7 nuclease, the supernatant containing neutrophil elastase is added to a substrate, which is selectively cleaved by elastase to yield a 4-nitroaniline product that adsorbs light at 405 nm. Enough reagents are provided to test 24 sample conditions for NET production, with analysis in duplicate. |
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| Size | 1 ea |
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| Concentration | n/a |
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| Applications | n/a |
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| Other Names | Neutrophil Extracellular Trap kit cell assay measuring measurement measure cell-based CBA neutrophil role engulfment destruction invasive microbe microorganism immune immunity system disease absence dysfunction antimicrobial response trap NET DNA deoxyribonuclease histone macromolecule bacterial plasma DNAse macrophage scavenger receptor-mediated phagocytosis NET/neutrophil complexe component myeloperoxidase MPO elastase NEs lactotransferrin peptide fluorescent fluorescence DNA-intercalating intercalating intercalate dye generation cellular injury injuries necrosis enzyme enzymatic S7 nuclease PMA ionophore A23187 NET-associated associated substrate cleaved cleavages formation NETosis |
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| Gene, Accession, CAS # | n/a |
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| Catalog # | 601010-1EA |
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| Order / More Info | NETosis Assay Kit from CAYMAN CHEMICAL COMPANY |
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| Product Specific References | n/a |
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