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产品所有类目:   透析，脱盐和缓冲Ac->>脱盐柱>>zeba自旋脱盐柱，7K 截留分子量>>
产品中文名称：zeba自旋脱盐柱，7K 截留分子量
产品英文名称：zeba spin desalting columns, 7k mwco
货号：pro_106256      产地：strem      规格/价格：询价
产品详细说明:
Zeba Spin Desalting Columns 7K MWCO

Fast and effective protein desalting for tiny and large volume samples (2µL to 4mL) with any standard benchtop centrifuge.

Zeba Desalting Spin ColumnsThermo Scientific Zeba Spin Desalting Columns 7K MWCO are polypropylene centrifuge columns containing high-performance Zeba Desalting Resin which offers rapid and exceptional protein desalting with high recovery. Available in 5 different columns sizes Zeba Spin Desalting Columns provide trouble-free desalting and buffer exchange for sample volumes ranging from 2µL to mL.

The easy-to-use spin column format dramatically improves results over standard drip-column methodologies eliminating the need to wait for samples to emerge by gravity flow and the need to monitor fractions for protein recovery. Zeba Desalting Columns require no chromatographic system cumbersome column preparation or equilibration and allow multiple samples to be processed in less than ten minutes.

Highlights of Zeba Spin Desalting Columns 7K MWCO:
  • Exceptional protein recovery
  • No screening fractions for protein or waiting for protein to emerge by gravity-flow
  • Wide product offering accommodates samples 2µL to 4mL
  • Easy-to-use with no cumbersome column preparation or equilibration
  • Minimal sample dilution

Colum sizes and recommended sample volumes.
Column Size Resin Bed Sample Size
Micro 75µL 2 - 12µL
0.5mL0.5mL30 - 130µL
2mL2mL200 - 700µL
5mL5mL500 - 2000µL
10mL10mL1500 - 4000µL

About Zeba Desalting Spin Columns

Zeba Spin Desalting Columns contain a proprietary high-performance desalting resin that offers exceptional desalting and protein-recovery characteristics compared to other commercially available resins. Even very dilute (25µg/mL) protein samples can be successfully processed to obtain greater than 95% retention (removal) of salts and other small molecules (< 1000 MW) and good recovery of proteins and other macromoecules (> 7000 MW).

Despite claims other commercially available resins perform only satisfactorily in spin columns with sample of high concentration (>250µg/mL) over a narrow window of sample volumes. The proprietary Zeba Micro Spin Desalting Columns has exceptional protein recovery and desalting characteristics with >95% retention of salts and other small molecules of <1000 MW and are recommended for processing compounds >7000 MW.

Zeba Desalt Spin Columns Figure 1

Increased protein recovery with Zeba Spin Desalting Columns. Samples of bovine serum albumin (BSA) 250µg/mL (A) and 25µg/mL (B) in 1M NaCl were desalted with 2mL Zeba Spin Desalting Columns and equivalent-sized colmns from two other suppliers (Comp. B and G). The recovered samples (10µL) were analyzed by SDS-PAGE conductivity and the BCA Protein Assay (Product #23225) to determine salt and protein concentrations respectively. Zeba Columns provided significantly greater protein recovery under all conditions tested. Conductivity and protein recovery values after desalting are indicated for 250µg/mL samples.

Zeba Mico Desalting Columns Effect of sample volume on protein recovery. Samples of bovine serum albumin at 1mg/mL were desalted with a Zeba Micro Desalting Column. Recovery percentages were analyzed by BCA Protein Assay (Product # 23225). Sample volumes were 3&181;L or 10µL with a 3µL buffer stacker placed over the sample.

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Ordering Information
Product #DescriptionPkg. SizeInstructionsMSDSCofAPrice
89877Zeba Micro Spin Desalting Columns 7K MWCO 0.075mL
for 2-12µL samples
25 columns

89878Zeba Micro Spin Desalting Columns 7K MWCO 0.075mL
for 2-12µL samples
50 columns

89882Zeba Spin Desalting Columns 7K MWCO 0.5mL
for 30–130uL samples
25 columns

89883Zeba Spin Desalting Columns 7K MWCO 0.5mL
for 30-130µL samples
50 columns

89889Zeba Spin Desalting Columns 7K MWCO 2mL
for 200-700µL samples
5 columns

89890Zeba Spin Desalting Columns 7K MWCO 2mL
for 200-700µL samples
25 columns /td>

89891Zeba Spin Desalting Columns 7K MWCO 5mL
for 500-2000µL samples
5 columns

89892Zeba Spin Desalting Columns 7K MWCO 5mL
for 500-2000µL samples
25 columns MSDS for product #89892 Zeba Spin Desalting Columns 7K MWCO 5mL

89893Zeba Spin Desalting Columns 7K MWCO 10mL
for 1500-4000µL samples
5 columns

89894Zeba Spin Desalting Columns 7K MWCO 10mL
for 1500-4000µL samples
25 columns