Thermo Scientific EZ-Link NHS-PEG4-Biotin is a PEGylated water-soluble reagent for simple and efficient biotin labeling of antibodies proteins and other primary amine-containing macromolecles.

The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. The hydrophilic polyethylene glycol (PEG) spacer arm of this biotinylation reagent imparts water solubility that is transferred to the biotinylated molecule thus reducing aggregation of labeled proteins stored in solution (). The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules.

Highlights:

• Label almost any protein with biotin to facilitate immobilization purification or detection
• Amine-reactive NHS ester reacts rapidly with any primary amine-containing molecle to attach the biotin label via a stable amide bond
• Reagent is water soluble and membrane impermeable – suitable for cell surface labeling
• PEGylation increases solubility and helps prevent aggregation of labeled molecules
• Long spacer arm reduces steric hindrance when binding to avidin molecules
• No-Weigh Format saves time preserves reagent activity and prevents cross-contamination between uses
• Reactive group: N-hydroxysuccinimide ester (NHS ester)
• Reactive toward: primary amines as in side chain of lysine (K) and N-terminus of polypeptides

Product Details:Properties of Thermo Scientific EZ-Link NHS-PEG-Biotin Labeling Reagents.

	NHS-PEG4-Biotin
Alternate names	NHS-PEO4-Biotin NHS-dPEG4-Biotin	NHS-PEO12-Biotin NHS-dPEG12-Biotin
Molecular weight	588.67	941.09
Spacer arm length	29 Å	56 Å
Mass added to target	474.59 daltons	825.64 daltons
Storage conditions	4°C with desiccant	-20°C with desiccant

 

Reaction scheme for Thermo Scientific EZ-Link NHS-Biotin Reagents. This example features the related reagent Sulfo-NHS-LC-ReagentPart No. 21335.

General References:

1. Altin J.G. et al. (1995. A one-step procedure for biotinylation and chemical crosslinking of lymphocyte surface and intracellular membrane-associated molecules. Anal. Biochem. 224 382-9.
2. Gretch D.R. et al. (1987) The use of biotinylated monoclonal antibodies and streptavidin affinity chromatography to isolate herpes virus hydrophobic proteins or glycoproteins. Anal. Biochem. 163 270-7.
3. Manning J. et al. (1977) A method for gene enrichment based on the avidin-biotin interaction. Application to the Drosophila ribosomal RNA genes. Biochemistry. 16 1364-70.
4. Updyke T.V. and Nicolson G.L. (1984) Immunoaffinity isolation of membrane antigens with biotinylated monoclonal antibodies and immobilized streptavidin matrices. J. Immunol. Meth. 73 83-95.

Product References:

1. Luo Y. et al. (2003) Mol. Cell. Biol. 23 2600-7.
2. Nielsen P.A. et al. (2002) J. Biol. Chem. 277 38272-83.
3. Xu G. et al. (2004) J. Biol. Chem. 117 3207-3219.
4. Vaidya V.S. et al. (2006) Am. J. Physiol. in press.
5. Nikitina T. and Woodcock C.L. (2004) J. Cell Biol. 166 161-165.

Related Products:

– very long (56.0Å) spacer arm variant
– 13.5Å spacer arm
– 22.4Å spacer arm
– 13.5Å spacer arm cleavable

Related Resources: