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产品中文名称:scai(r0122),限制性内切酶
产品英文名称:scai(r0122), restriction endonucleases
货号:pro_50963      产地:neb      规格/价格:询价
产品详细说明:
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scai
scai has a available
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recognition site:

agtact

    

description:
scai has a high fidelity version scai-hf™ ().

have been engineered for reduced star activity and have 100% activity in buffer 4 which may simplify your double digest.

source:
an e. coli strain that carries the scai gene from streptomyces caespitosus (h. takahashi).

reagents supplied:
nebuffer 3


enzyme properties


activity in nebuffers:
nebuffer 1:not recommended
nebuffer 2:not recommended
nebuffer 3:100%
nebuffer 4:not recommended

when using a buffer other than the optimal (supplied) nebuffer, it may be necessary to add more enzyme to achieve complete digestion

methylation sensitivity:
dam methylation: not sensitive
dcm methylation: not sensitive
cpg methylation: not sensitive
more information about:

heat inactivation:
80°c for 20 minutes

survival in a reaction:
(+ +) intermediate activity. suitable for extended digestion, but <8 hours.
more information about:


reaction & storage conditions


reaction conditions:
1x nebuffer 3
incubate at 37°c.

1x nebuffer 3:
50 mm tris-hcl
100 mm nacl
10 mm mgcl2
1 mm dithiothreitol
ph 7.9 @ 25°c

unit definition:
one unit is defined as the amount of enzyme required to digest 1 ug of λλna in 1 hour at 37°c in a total reaction volume of 50ul.

concentration:
10,000 units/ml and 50,000 units/ml

unit assay substrate:
λ dna

storage conditions:
10 mm tris-hcl
50 mm kcl
1 mm dithiothreitol
0.1 mm edta
200 µg/ml bsa
50% glycerol
ph 7.4 @ 25°c

storage temperature:
-20°c

diluent compatibility:
diluent a


notes


general notes:
  1. conditions of low ionic strength, high enzyme concentration, glycerol concentration> 5%, or ph> 8.0 may result in star activity.
  2. to avoid star activity, do not use this enzyme in nebuffer 1, nebuffer 2 or nebuffer 4.