| 产品所有类目: 限制性内切酶>>限制性内切酶>>scai>> | 产品中文名称:scai(r0122),限制性内切酶 | 产品英文名称:scai(r0122), restriction endonucleases | 货号:pro_50963 产地:neb 规格/价格:询价 | 产品详细说明: | > > > > scai |  |  |  |  |  |  |  |  |  |  |  |  |  |  | catalog # |  | size |  | concentration |  | price |  | qty |  | |  |  |  |  |  |  |  | | | download: |  | |  |

 recognition site:



description: scai has a high fidelity version scai-hf™ ().
have been engineered for reduced star activity and have 100% activity in buffer 4 which may simplify your double digest.
source: an e. coli strain that carries the scai gene from streptomyces caespitosus (h. takahashi).
reagents supplied: nebuffer 3
enzyme properties

 activity in nebuffers:
nebuffer 1: |  | not recommended | nebuffer 2: |  | not recommended | nebuffer 3: |  | 100% | nebuffer 4: |  | not recommended |
when using a buffer other than the optimal (supplied) nebuffer, it may be necessary to add more enzyme to achieve complete digestion
methylation sensitivity:
dam methylation: not sensitive | dcm methylation: not sensitive | cpg methylation: not sensitive | more information about: |
heat inactivation: 80°c for 20 minutes
survival in a reaction: (+ +) intermediate activity. suitable for extended digestion, but <8 hours. more information about:
reaction & storage conditions

 reaction conditions: 1x nebuffer 3 incubate at 37°c.
1x nebuffer 3: 50 mm tris-hcl 100 mm nacl 10 mm mgcl2 1 mm dithiothreitol ph 7.9 @ 25°c
unit definition: one unit is defined as the amount of enzyme required to digest 1 ug of λλna in 1 hour at 37°c in a total reaction volume of 50ul.
concentration: 10,000 units/ml and 50,000 units/ml
unit assay substrate: λ dna
storage conditions: 10 mm tris-hcl 50 mm kcl 1 mm dithiothreitol 0.1 mm edta 200 µg/ml bsa 50% glycerol ph 7.4 @ 25°c
storage temperature: -20°c
diluent compatibility: diluent a
notes

 general notes:- conditions of low ionic strength, high enzyme concentration, glycerol concentration> 5%, or ph> 8.0 may result in star activity.
- to avoid star activity, do not use this enzyme in nebuffer 1, nebuffer 2 or nebuffer 4.
|
|