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heat inactivation: 65°c for 20 minutes survival in a reaction: (+ +) intermediate activity. suitable for extended digestion, but <8 hours. more information about: reaction & stoage conditions reaction conditions: 1x nebuffer 4 incubate at 37°c. 1x nebuffer 4: 20 mm tris-acetate 50 mm potassium acetate 10 mm magnesium acetate 1 mm dithiothreitol ph 7.9 @ 25°c unit definition: one unit is defined as the amount of enzyme required to digest 1 ug of λλna in 1 hour at 37°c in a total reaction volume of 50ul. concentration: 10,000 units/ml λ dna storage conditions: 10 mm tris-hcl 50 mm nacl 1 mm dithiothreitol 0.1 mm edta 200 µg/ml bsa 50% glycerol ph 7.4 @ 25°c storage temperature: -20°c diluent compatibility: diluent a notes general notes:
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