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产品中文名称:bspdi(r0557),限制性内切酶
产品英文名称:bspdi(r0557), restriction endonucleases
货号:pro_50952      产地:neb      规格/价格:询价
产品详细说明:
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bspdi
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recognition site:

atcgat

    

source:
bacillus species (h. kong)

reagents supplied:
nebuffer 4


enzyme properties


activity in nebuffers:
nebuffer 1:25%
nebuffer 2:75%
nebuffer 3:50%
nebuffer 4:100%

when using a buffer other than the optimal (supplied) nebuffer, it may be necessary to add more enzyme to achieve complete digestion.

methylation sensitivity:
dam methylation: blocked by overlapping
dcm methylation: not sensitive
cpg methylation: blocked
more information about:

heat inactivation:
65°c for 20 minutes

survival in a reaction:
(+ +) intermediate activity. suitable for extended digestion, but <8 hours.
more information about:


reaction & stoage conditions


reaction conditions:
1x nebuffer 4
incubate at 37°c.

1x nebuffer 4:
20 mm tris-acetate
50 mm potassium acetate
10 mm magnesium acetate
1 mm dithiothreitol
ph 7.9 @ 25°c

unit definition:
one unit is defined as the amount of enzyme required to digest 1 ug of λλna in 1 hour at 37°c in a total reaction volume of 50ul.

concentration:
10,000 units/ml
unit assay substrate:
λ dna

storage conditions:
10 mm tris-hcl
50 mm nacl
1 mm dithiothreitol
0.1 mm edta
200 µg/ml bsa
50% glycerol
ph 7.4 @ 25°c

storage temperature:
-20°c

diluent compatibility:
diluent a


notes


general notes:
  1. bspdi is an isoschizomer of clai./span>