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产品所有类目:   限制性内切酶>>限制性内切酶>>alei>> 产品中文名称：alei（r0634），限制性内切酶 产品英文名称：alei(r0634), restriction endonucleases 货号：pro_50928      产地：neb      规格/价格：询价 产品详细说明: > > > > alei alei catalog # size concentration price qty   download: recognition site:      source: an e. coli strain that carries the cloned alei gene aureobacterium liquefaciens (s.k. degtyarev) reagents supplied: nebuffer 4 enzyme properties activity in nebuffers: 10% nebuffer 2: 25% nebuffer 3: 10% nebuffer 4: 100% when using a buffer other than the optimal (supplied) nebuffer, it may be necessary to add more enzyme to achieve complete digestion methylation sensitivity: dam methylation: not sensitive dcm methylation: not sensitive cpg methylation: impaired by some combinations of overlapping more information about: heat inactivation: 65°c for 20 minutes survival in a reaction: (+ +) intermediate activity. suitable for extended digestion, but <8 hours. more information about: reaction & storage conditions reaction conditions: 1x nebuffer 4 incubate at 37°c. 1x nebuffer 4: 20 mm tris-acetate 50 mm potassium acetate 10 mm magnesium acetate 1 mm dithiothreitol ph 7.9 @ 25°c unit definition: one unit is defined as the amount of enzyme required to digest 1 ug of λ dna in 1 hour at 37°c in a total reaction volum of 50ul. concentration: 10,000 units/ml unit assay substrate: λ dna storage conditions: 20 mm tris-hcl 300 mm nacl 1 mm dithiothreitol 0.1 mm edta 200 µg/ml bsa 50% glycerol ph 7.5 @ 25°c storage temperature: -70°c diluent compatibility: diluent a notes general notes: alei is an isoschizomer of olii.