※产品导航	产品所有类目:   限制性内切酶>>切口内切酶>>nb.bbvci>> 产品中文名称：nb.bbvci（r0631），切口内切酶 产品英文名称：nb.bbvci(r0631), nicking endonucleases 货号：pro_50899      产地：neb      规格/价格：询价 产品详细说明: > > > > nb.bbvci nb.bbvci catalog # size concentration price qty   download: recognition site:    description: nb.bbvci is a nicking endonuclease that cleaves only one strand of dna on a double-stranded dna substrate. source: an e. coli strain expressing an altered form of the bbvci restriction genes [ra+:rb(e177g)] from bacillus brevis (l. ge) reagents supplied: nebuffer 2 enzyme properties activity in nebuffers: nebuffer 1: 50% nebuffer 2: 100% nebuffer 3: 10% nebuffer 4: 100% when using a buffer other than the optimal (supplied) nebuffer, it may be necessary to add moreenzyme to achieve complete digestion. methylation sensitivity: dam methylation: not sensitive dcm methylation: not sensitive cpg methylation: not sensitive more information about: heat inactivation: 80°c for 20 minutes survival in a reaction: (+ + +) suitable for an extended or overnight digestion. enzyme is active> 8 hours. more information about: reaction & storage conditions reaction conditions: 1x nebuffer 2 incubate at 37°c. 1x nebuffer 2: 10 mm tris-hcl 50 mm nacl 10 mm mgcl2 1 mm dithiothreitol ph 7.9 @ 25°c unit definition: one unit is defined as the amount of enzyme required to convert 1 ug of supercoiled plasmid dna to open circular form in 1 hour at 37&176;c in a total reaction volume of 50ul. concentration: 10,000 units/ml unit assay substrate: supercoiled plasmid dna storage conditions: 10 mm tris-hcl 50 mm kcl 1 mm dithiothreitol 0.1 mm edta 200 µg/ml bsa 50% glycerol ph 7.4 @ 25°c storage temperature: -20°c diluent compatibility: diluent a quality control for current lot quality control values for a specific lot can be found on the datacard which accompanies each product. 16-hour incubation: a 50 μl reaction containing 1 μg of dna and 30 units of nb.bbvci incubated for 16 hours at 37ºc resulted in a dna pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. exonuclease activity: incubation of a 50 μl reaction containing 30 units of nb.bbvci with 1 μg of a mixture of single and double-stranded [3h] e. coli dna (105 cpm/μg) for 4 hours at 37ºc released <0.05% of the total radioactivity. references song, q. et al. (2010) anal. chem, [epub ahead of print]. zhang, p. et al. (2010) protein expr. purif, 69, 226-234. [epub 2009 sep 9]. reagents sold separately companion products