Clontech offers a wide variety of monoclonal and polyclonal antibodies for fluorescent protein detection. Whether you are working with Aequorea victoria (EGFP) or Aequorea coerulescens (AcGFP1), with one of our Reef Coral Fluorescent Proteins such as DsRed or HcRed, or with one of our Fruit Fluorescent Proteins, we have an antibody to fit your needs (Tables I and II). All of our antibodies are carefully tested to verify their specificities and ensure their lot-to-lot performance.

Red Fluorescent Protein & Fruit Fluorescent Protein Antibodies

mCherry Monoclonal Antibody: The Living Colors mCherry Monoclonal was raised against mCherry. It recognizes mCherry, mOrange, mOrange2, mPlum, and mStrawberry, as well as C- and N-terminal fusions containing these fluorescent proteins. The mCherry Monoclonal Antibody can be used to differentiate mCherry (which it detects) from DsRed-Express and/or tdTomato (which it does not).

DsRed Antibodies: The DsRed Antibodies recognize wild-type Discosoma sp. red fluorescent protein (DsRed) and most variants, including DsRed1, DsRed2, the Fluorescent Timer (DsRed1-E5), DsRed-Express, and DsRed-Express2, although only the DsRed Polyclonal antibody is used for detection of the DsRed-Monomer.  Both C-terminal and N-terminal fusions to the red fluorescent protein can be detected. On Western blots, these antibodies exhibit no cross-reactivity with A.v. GFP or its variants or with HcRed, the far-red fluorescent protein from Heteractis crispa (Figure 2). The DsRed Polyclonal Antibody can be used at high dilutions (1:1,000) in Western blots.

The DsRed Monoclonal ad Polyclonal Antibodies can also be used to detect the Fruit Fluorescent Proteins by Western blot. The mCherry Monoclonal Antibody can be used to differentiate mCherry (which it detects) from DsRed-Express and/or tdTomato (which it does not).

HcRed Polyclonal Antibody: The HcRed Polyclonal Antibody was raised against a peptide of HcRed1, a far-red fluorescent protein whose wild-type cDNA is derived from the Anthozoa-class sea anemone Heteractis crispa. The antibody has been purified using an antigen peptide affinity column. It recognizes denatured HcRed1 as well as both N- and C-terminal fusion proteins containing HcRed1 in bacterial and mammalian cell lysates. The antibody is recommended for Western blot analysis. It displays cross-reactivity to Discosoma sp. red fluorescent protein (DsRed) and the enhanced GFP colr variants from Aequorea victoria.

Green Fluorescent Protein Antibodies

EGFP Monoclonal Antibody: This antibody recognizes native and denatured forms of Aequorea victoria EGFP (enhanced green fluorescent protein), EGFP fusions, and destabilized EGFP variants. The EGFP Monoclonal Antibody is raised against full-length enhanced GFP. Its uses include immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, and western blots. Click here to learn more about the EGFP Monoclonal Antibody

GFP Monoclonal Antibody: A monoclonal antibody produced by hybridoma cellsagainst recombinant full-length Aequorea victoria green fluorescent protein (rGFP). This antibody recognizes wild-type GFP, GFPuv, EGFP, destabilized EGFP variants, EBFP, EYFP, ECFP and both N- and C-terminal fusion proteins containing these GFP variants in bacterial and mammalian cell lysates.

ZsGreen Polyclonal Antibody: The Full Length ZsGreen Polyclonal Antibody was raised in rabbit against recombinant full length ZsGreen1 protein. This polyclonal antibody specifically detects Zoanthus sp. green fluorescent protein (ZsGreen), and can be used to identify and confirm fusion constructs in bacteria and in mammalian cells. It has high specificity to ZsGreen, and does not cross-react with other fluorescent proteins, including other Reef Coral Fluorescent Proteins (RCFPs) or green fluorescent protein variants derived from Aequorea species (including AcGFP1).

Othr Green Fluorescent Protein Antibodies: The A.v. GFP Antibodies recognize wild-type Aequorea victoria green fluorescent protein (GFP) and AcGFP1 from Aequorea coerulescens. These antibodies recognize N- and C-terminal fusions to these proteins as well as destabilized variants, which are commonly used to measure promoter activity.

• A.v. Monoclonal Antibody (JL-8) - Raised against full-length Aequorea victoria green fluorescent protein (GFP).
• Full-Length A.v. Polyclonal Antibody - Raised against recombinant full-length Aequorea victoria green fluorescent protein (rGFP).
• A.v. Peptide Antibody - A mixture of several rabbit polyclonal antibodies that were raised against synthetic peptides corresponding to the central and C-terminal portions of Aequorea victoria green fluorescent protein (GFP).

Ref Coral Antibody

Anti-RCFP Polyclonal Pan Antibody: The Anti-RCFP Polyclonal Pan Antibody detects any of the entire panel of Reef Coral Fluorescent Proteins. It was raised in rabbit against recombinant full-length Zoanthus sp. yellow fluorescent protein (ZsYellow). This antibody recognizes AmCyan, ZsGreen, ZsYellow, DsRed2, DsRed-Express, HcRed, and AsRed in Western blot analyses. Just as importantly, it does not recognize green fluorescent protein variants derived from Aequorea species (including AcGFP1). This polyclonal antibody can detect fusion constructs and thus can be used to identify and confirm fusion proteins in bacteria and in mammalian cells.

Figure 2. Western blot detection of red fluorescent proteins: 

• Panel A. Use the DsRed Monoclonal antibody to detect DsRed variants except DsRed-Monomer (and to detect the Fruit Fluorescent Proteins; data not shown). Hel.a cells were transiently transfected with the following vectors, and then analyzed by Western blot with the DsRed Monoclonal Antibody. Lane 1: pDsRed2-N1, Lane 2: pDsRed2-C1, Lane 3: pDsRed1-C1, Lane 4: pDsRed2-Mito (N-terminal fusion), Lane 5: pDsRed2-Nuc (C-terminal fusion), Lane 6: control (untransfected cells). Note that a 20 kDa, non-specific band appears with extended exposure. 
• Panel B. Use the DsRed Polyclonal antibody to detect any DsRed variant (including DsRed-Monomer) and to detect the Fruit Fluorescent Proteins; data not shown. Western blot with the DsRed Polyclonal Antibody.  Lane 1: 25 ng of recombinant AcGFP1 protein.  Lanes 2-5: HEK-293 cells were stably transfected with the following vectors Lane 2: pAcGFP1. Lane 3: Control (untransfected cells). Lane 4: pDsRed-Monomer-N1. Lane 5: pDsRed-Express-N1.
• Panel C. Use the mCherry Monoclonal Antibody to detect mCherry, but not tdTomato, DsRed-Express2, or DsRed-Monomer. HEK293 cells were transiently transfected with vectors encoding the following fluorescent proteins, and then analyzed by Western blot with the mCherry Monoclonal Antibody. Lane 1: control (untransfected cells), Lane 2: mCherry, Lane 3: tdTomato, Lane 4: DsRed-Monomer, Lane 5: DsRed-Express2.
• Panel D. Use the mCherry Monoclonal Antibody to differentiate between Fruit Fluorescent Proteins. The mCherry Monoclonal Antibody detects mCherry, mOrange, mPlum, and mStrawberry, but not mRaspberry or tdTomato. mBanana is only faintly detected. HEK293 cells were transiently transfected with vectors encoding the following fluorescent proteins, and then analyzed by Western bot with the mCherry Monoclonal Antibody. Lane 1: control (untransfected cells), Lane 2: mCherry, Lane 3: mBanana, Lane 4: mOrange, Lane 5: mPlum, Lane 6: mRaspberry, Lane 7: mStrawberry, Lane 8: tdTomato.