Kit Summary:
? Detection method- Absorbance (450 nm)
? Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
? Species reactivity- Mammalian

Features & Benefits:
? Simple procedure; takes ~1 hour
? Fast and convenient
? Kit contains the necessary reagents for measurement of cholesterol in various biological samples

Kit components:
? Cholesterol Assay Buffer
? Substrate Mix (lyophilized)
? Enzyme Mix (lyophilized)
? Esterase (lyophilized)
? Cholsterol Standard (2 ?g/?l)

Description:
Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases. The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method. Most of the cholesterol in blood is in the form of cholesteryl ester. These esters can be hydrolyzed to free cholesterol under the appropriate conditions. In the assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm. The assay can detect free or total cholesterol depending upon whether esterase is utilized to hydrolyze cholesterol esters present. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters). The probe in this kit is more stable, sensitive and specific. The asay can tolerate interferences from various samples significantly.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.