High Yield Transcription Kit for dsRNA Preparation
The MEGAscript RNAi Kit is optimized for the synthesis of large mass amounts of ready-to-use dsRNA for RNAi experiments in non-mammalian systems. The MEGAscript RNAi Kit is based on Ambion′s patented high-yield transcription technology and includes reagents fo transcripton,
nuclease digestion and clean up.Synthesize up to 2 mg of dsRNA
The ability to generate large amounts of clean,
double-stranded RNA is essential for the success of RNAi experiments. The MEGAscript RNAi Kit,
based on Ambion′s patented MEGAscript large scale transcription technology allows RNAi researchers to synthesize 50 to 100 µg or more of double-stranded RNA in a single transcription reaction. The yield is typically 10-50 times the amount of RNA produced by conventional transcription reactions. DNA of either PCR or plasmid origin can be used as a template for the transcription reaction. The sense and antisense strands can be synthesized from a PCR generated DNA template containing T7 RNA Polymerase promoters on both ends of the template,
eliminating the need for a separate annealing step. dsRNA Ready for Injection
The MEGAscript RNAi Kit provides all the reagents necessary to eliminate DNA template,
single-stranded RNA (ssRNA),
and fre nucleotidesfrom the dsRNA preparation. After the dsRNA is synthesized,
it is digested with DNase I and single strand specific RNase (included) to degrade the DNA template and any residual ssRNA. The dsRNA is then purified away from proteins,
free nucleotides,
and nucleic acid degradation products using a quick glass fiber filter-based procedure. The MEGAscript RNAi Kit comes with a control double-stranded cDNA template corresponding to a portion of the Drosophila melanogaster nautilus gene for assessing the proper function of the kit. A comprehensive Instruction Manual with a detailed troubleshooting section is also provided. | 