RFC4 Antibody is a Rabbit Polyclonal antibody against RFC4. The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported.
| Target |
RFC4 |
| Reactivity |
Human, Rat |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Tested Applications |
WB, IHC, IF/ICC, IP |
| Recommended dilutions |
WB: 1/500 - 1/2000, IHC: 1/50 - 1/200, IF/ICC: 1/50 - 1/100, IP: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Recombinant protein of human RFC4. |
| Purification |
Affinity purified. |
| Form |
Liquid |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Molecular Weight |
Calculated MW: 33 kDa/39 kDa
Observed MW: 38 kDa |
| Swiss Prot |
P35249
|
| GeneID |
5984
|
| Gene Symbol |
RFC4 |
| Concentration |
> 1 mg/ml |
| Buffer |
PBS, pH 7.3, 0.02% sodium azide, 50% glycerol. |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |