Oct4: octamer-binding transcription factor-4 (Oct-4, Otf-4) and Oct-3/4, also known as POU5F1 (POU class 5 homeobox 1), octamerbinding transcription factor-3 (Oct-3, Otf-3), modulates embryonic stem (ES) cell populations by influencing lineage commitment.Entrez Protein NP_002692.Oct-3/4 sustains stem-cell selfrenewal and differentiation pathways.Transcription factors containing the POU homeodomain regulate tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development.Oct-3/4 is capable of inducing rapid proliferation and tumorigenic properties of ES cells through activation of the UTF1 gene.In humans, two Oct-3/4 isoforms contribute to influencing the undifferentiated phenotype of ES cells.Oct-3/4 pseudogenes localizing to human chromosomes 10 and 8 are reported to be transcribed in certain cancer cell lines and tissues.
| Target |
POU Class 5 Homeobox 1 (OCT4/POU5F1) |
| Reactivity |
Human |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
ELISA, WB |
| Recommended dilutions |
ELISA: 1/10000, WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Purified recombinant fragment of Oct4 (aa193-360) expressed in E. coli. |
| Purification |
Unpurified Ascites. |
| Isotype |
IgG1 |
| Conjugation |
Unconjugated |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Molecular Weight |
38.5 kDa |
| Swiss Prot |
Q01860
|
| GeneID |
5460
|
| Gene Symbol |
POU5F1 |
| OMIM |
164177
|
| HGNC |
9221 |
| Ensembl |
ENSG00000204531 |
| Buffer |
Ascitic fluid containing 0.03% sodium azide. |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |