Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes.All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids.These include the POU homeodomain and the POU-specific domain.Both are required for DNA binding and are involved in protein-protein interactions.Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.Oct4 is renowned as a transcription factor expressed by undifferentiated embryonic stem cells and embryonic germ cells.
| Target |
POU Class 5 Homeobox 1 (OCT4/POU5F1) |
| Reactivity |
Human |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
ELISA, WB |
| Recommended dilutions |
ELISA: 1/10000, WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Purified recombinant fragment of OCT4 expressed in E. coli. |
| Purification |
Unpurified Ascites. |
| Isotype |
IgG1 |
| Conjugation |
Unconjugated |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Molecular Weight |
39 kDa |
| Swiss Prot |
Q01860
|
| GeneID |
5460
|
| Gene Symbol |
POU5F1 |
| OMIM |
164177
|
| HGNC |
9221 |
| Ensembl |
ENSG00000204531 |
| Buffer |
Ascitic fluid containing 0.03% sodium azide. |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |