BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109.Entrez Protein NP_004324.It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway.B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439).B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region.Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras.Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers.The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
| Target |
BRAF |
| Reactivity |
Human, Mouse |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Tested Applications |
ELISA, WB, IHC, IF/ICC |
| Recommended dilutions |
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, IF/ICC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Immunogen |
Purified recombinant fragment of human BRAF expressed in E. coli. |
| Purification |
Unpurified Ascites. |
| Isotype |
IgG1 |
| Conjugation |
Unconjugated |
| Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Molecular Weight |
87 kDa |
| GeneID |
673
|
| Buffer |
Ascitic fluid containing 0.03% sodium azide. |
| UNSPSC Code |
12352203 |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |