SHC1 (pY349) Antibody is a Rabbit Polyclonal antibody against SHC1 (pY349). This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
Target |
SHC1 (pY349) |
Reactivity |
Human, Mouse |
Host |
Rabbit |
Clonality |
Polyclonal |
Tested Applications |
WB, IF/ICC |
Recommended dilutions |
WB: 1/500 - 1/2000, IF/ICC: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
Immunogen |
Synthetic Peptide. A phospho specific peptide corresponding to residues surrounding Y349 of human SHC1. |
Purification |
Affinity purified. |
Form |
Liquid |
Isotype |
IgG |
Conjugation |
Unconjugated |
Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
Molecular Weight |
Calculated MW: 40 kDa/46 kDa/51 kDa/62 kDa Observed MW: 50 kDa |
Swiss Prot |
P29353
|
GeneID |
6464
|
Gene Symbol |
SHC1 |
Concentration |
> 1 mg/ml |
Buffer |
PBS, pH 7.3, 0.02% sodium azide, 50% glycerol. |
UNSPSC Code |
12352203 |
Availability |
Shipped within 5-10 working days. |
Note |
This product is for research use only. |