产品名称 Sialic Acid Binding Ig Like Lectin 5 (SIGLEC5) Antibody
产品货号 Catalogue No: abx028681
产品价格 现货询价,电话:010-67529703
产品规格 Available Options * Size: 80 µl 400 µl
产品品牌 abbexa
产品概述
产品详情
SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have one or more cytoplasmic immune receptor tyrosine-based inhibitory motifs (ITIM). SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and four C2-set Ig-like domains, five potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8. Expression was also detected on monocytes and a CD16-positive/CD56-negative natural killer-like lymphocyte population. After sialidase treatment, which is necessary for unmasking the sialic acid-binding site on SIGLECs interacting with cell surface sialic acids, cells expressing SIGLEC10 bound to red blood cells. Immunoprecipitation analysis indicated expression of a 100 to 120-kD monomeric protein, higher than the predicted molecular mass, suggesting that SIGLEC10 is glycosylated.
Target SIGLEC5
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Tested Applications WB
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Immunogen KLH-conjugated synthetic peptide between 482-513 amino acids from the C-terminal region of human SIGLEC5.
Purification Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Isotype IgG
Conjugation Unconjugated
Storage Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Swiss Prot O15389
Gene Symbol SIGLEC5
Buffer PBS containing 0.09% sodium azide.
UNSPSC Code 12352203
Availability Shipped within 5-10 working days.
Note This product is for research use only.
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